Efficacy of Silicone Conduit in the Rat Sciatic Nerve Repair Model: Journey of a Thousand Miles.
Anastomosis
conduit
electron microscopic
glue
rat nerve model
sciatic
sciatic functional index
Journal
Neurology India
ISSN: 1998-4022
Titre abrégé: Neurol India
Pays: India
ID NLM: 0042005
Informations de publication
Date de publication:
Historique:
entrez:
27
4
2021
pubmed:
28
4
2021
medline:
22
6
2021
Statut:
ppublish
Résumé
A lot of options have been tried for bridging the two ends of the injured nerves. Researchers have used decellularized nerve grafts, artificial materials and even nerve growth factors to augment functional recovery. These materials are either costly or inaccessible in developing world. The study aimed to evaluate the efficacy of the silicone conduit in a rat sciatic nerve injury model. 24 healthy Sprague-Dawley (SD) rats (250-300 grams; 8-10 weeks) were used and right sciatic nerve was exposed; transected and re-anastomosed by two different methods in 16 rats. In control group, n = 8 (Group I) the sciatic nerve was untouched; Group II (reverse nerve anastomosis, n = 8): 1-centimeter of nerve was cut and re-anastomosed by using 10-0 monofilament suture; Group III (silicone conduit, n = 8) 1-centimeter nerve segment was cut, replaced by silicone conduit and supplemented by fibrin glue]. Evaluation of nerve recovery was done functionally (pain threshold and sciatic functional index) over 3 months and histologically and electron microscopically. Functional results showed a trend of clinical improvement in Group III and II but recovery was poor and never reached up to normal. Histopathological and electron microscopic results showed an incomplete axonal regeneration in Groups II and III. Psychological analyses showed that no outwards signs of stress were present and none of the rats showed paw biting and teeth chattering. The silicone conduit graft may be an economical and effective alternative to presently available interposition grafts, however for short segments only.
Sections du résumé
BACKGROUND
BACKGROUND
A lot of options have been tried for bridging the two ends of the injured nerves. Researchers have used decellularized nerve grafts, artificial materials and even nerve growth factors to augment functional recovery. These materials are either costly or inaccessible in developing world.
OBJECTIVE
OBJECTIVE
The study aimed to evaluate the efficacy of the silicone conduit in a rat sciatic nerve injury model.
MATERIALS AND METHODS
METHODS
24 healthy Sprague-Dawley (SD) rats (250-300 grams; 8-10 weeks) were used and right sciatic nerve was exposed; transected and re-anastomosed by two different methods in 16 rats. In control group, n = 8 (Group I) the sciatic nerve was untouched; Group II (reverse nerve anastomosis, n = 8): 1-centimeter of nerve was cut and re-anastomosed by using 10-0 monofilament suture; Group III (silicone conduit, n = 8) 1-centimeter nerve segment was cut, replaced by silicone conduit and supplemented by fibrin glue]. Evaluation of nerve recovery was done functionally (pain threshold and sciatic functional index) over 3 months and histologically and electron microscopically.
RESULTS
RESULTS
Functional results showed a trend of clinical improvement in Group III and II but recovery was poor and never reached up to normal. Histopathological and electron microscopic results showed an incomplete axonal regeneration in Groups II and III. Psychological analyses showed that no outwards signs of stress were present and none of the rats showed paw biting and teeth chattering.
CONCLUSION
CONCLUSIONS
The silicone conduit graft may be an economical and effective alternative to presently available interposition grafts, however for short segments only.
Identifiants
pubmed: 33904443
pii: ni_2021_69_2_318_314576
doi: 10.4103/0028-3886.314576
doi:
Substances chimiques
Silicones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
318-325Commentaires et corrections
Type : CommentIn
Déclaration de conflit d'intérêts
None