Effect of high phosphate diet on the formation of dentin in Fam20c-deficient mice.
FAM20C
dentin
high phosphate diet
hypophosphatemia
mouse
Journal
European journal of oral sciences
ISSN: 1600-0722
Titre abrégé: Eur J Oral Sci
Pays: England
ID NLM: 9504563
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
revised:
03
04
2021
received:
02
12
2020
accepted:
04
04
2021
pubmed:
28
4
2021
medline:
25
6
2021
entrez:
27
4
2021
Statut:
ppublish
Résumé
FAM20C (family with sequence similarity 20-member C), a kinase that phosphorylates secretory proteins, plays essential roles in various biological processes. In humans, mutations in FAM20C gene cause Raine syndrome, an autosomal recessive hereditary disease manifesting a broad spectrum of developmental defects including skeletal and craniofacial deformities. Our previous studies revealed that inactivation of Fam20c in mice led to hypophosphatemic rickets and that high phosphate (hPi) diet significantly improved the development of the skeleton in Fam20c-deficient mice. In this study, we evaluated the effects of hPi diet on the formation of dentin in Fam20c-deficient mice, using plain x-ray radiography, micro-computed tomography (µCT), histology, and immunohistochemistry. Plain x-ray radiography and µCT analyses showed that the hPi diet improved the dentin volume fraction and dentin mineral density of the Fam20c-deficient mice. Histology analyses further demonstrated that the hPi diet dramatically improved the integrity of the mandibular first molars and prevented pulp infection and dental abscesses in Fam20c-deficient mice. Our results support that the hPi diet significantly increased the formation and mineralization of dentin in Fam20c-deficient mice, implying that hypophosphatemia is a significant contributor to the dentin defects in Fam20c-deficient subjects.
Identifiants
pubmed: 33905141
doi: 10.1111/eos.12795
pmc: PMC8183185
mid: NIHMS1707517
doi:
Substances chimiques
Calcium-Binding Proteins
0
Extracellular Matrix Proteins
0
FAM20C protein, mouse
0
Phosphates
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12795Subventions
Organisme : NIDCR NIH HHS
ID : R01 DE022549
Pays : United States
Organisme : Texas A&M University College of Dentistry Department of Biomedical Sciences Seed Grant
ID : TAMCOD-BMS-2019-004 (to HZ)
Organisme : Foundation for the National Institutes of Health
ID : DE022549 (to CQ)
Informations de copyright
© 2021 European Journal of Oral Sciences.
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