Spatiotemporal Resolution of Conformational Changes in Biomolecules by Combining Pulsed Electron-Electron Double Resonance Spectroscopy with Microsecond Freeze-Hyperquenching.

Electrons Freezing Mesorhizobium / chemistry Models, Molecular Potassium Channels / chemistry Protein Conformation Spectrum Analysis Time Factors

Journal

Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056

Informations de publication

Date de publication:
12 05 2021
Historique:
pubmed: 28 4 2021
medline: 15 12 2021
entrez: 27 4 2021
Statut: ppublish

Résumé

The function of proteins is linked to their conformations that can be resolved with several high-resolution methods. However, only a few methods can provide the temporal order of intermediates and conformational changes, with each having its limitations. Here, we combine pulsed electron-electron double resonance spectroscopy with a microsecond freeze-hyperquenching setup to achieve spatiotemporal resolution in the angstrom range and lower microsecond time scale. We show that the conformational change of the C

Identifiants

DOI: 10.1021/jacs.1c01081 PMID: 33905249
pubmed: 33905249
doi: 10.1021/jacs.1c01081
doi:

Substances chimiques

Potassium Channels 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6981-6989

Auteurs

Tobias Hett (T)

Institute of Physical and Theoretical Chemistry, University of Bonn, Wegelerstraße 12, 53115 Bonn, Germany.
ORCID: 0000-0003-3821-5165

Tobias Zbik (T)

Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.

Shatanik Mukherjee (S)

Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.

Hideto Matsuoka (H)

Institute of Physical and Theoretical Chemistry, University of Bonn, Wegelerstraße 12, 53115 Bonn, Germany.

Wolfgang Bönigk (W)

Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.

Daniel Klose (D)

Fachbereich Physik, Universität Osnabrück, Barbarastraße 7, 49076 Osnabrück, Germany.
ORCID: 0000-0002-3597-0889

Christophe Rouillon (C)

Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.

Norbert Brenner (N)

Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.

Sebastian Peuker (S)

Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.

Reinhard Klement (R)

Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Heinz-Jürgen Steinhoff (HJ)

Fachbereich Physik, Universität Osnabrück, Barbarastraße 7, 49076 Osnabrück, Germany.

Helmut Grubmüller (H)

Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
ORCID: 0000-0002-3270-3144

Reinhard Seifert (R)

Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.

Olav Schiemann (O)

Institute of Physical and Theoretical Chemistry, University of Bonn, Wegelerstraße 12, 53115 Bonn, Germany.
ORCID: 0000-0001-6346-9779

U Benjamin Kaupp (UB)

Center of Advanced European Studies and Research (caesar), Ludwig-Erhard-Allee 2, 53175 Bonn, Germany.
Life & Medical Sciences Institute (LIMES), University of Bonn, Carl-Troll-Straße 31, 53115 Bonn, Germany.
ORCID: 0000-0002-0696-6397

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Phénomènes électromagnétiques › Électrons › Spatiotemporal Resolution of Conformational...
questionsmedicales.fr › Phénomènes chimiques › Transition de phase › Congélation › Spatiotemporal Resolution of Conformational...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Phyllobacteriaceae › Mesorhizobium › Spatiotemporal Resolution of Conformational...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Spatiotemporal Resolution of Conformational...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Canaux potassiques › Spatiotemporal Resolution of Conformational...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Spatiotemporal Resolution of Conformational...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spatiotemporal Resolution of Conformational...
questionsmedicales.fr › Phénomènes physiques › Temps › Facteurs temps › Spatiotemporal Resolution of Conformational...