Control of pre-replicative complex during the division cycle in Chlamydomonas reinhardtii.
Animals
Cell Cycle Proteins
/ genetics
Cell Division
/ genetics
Cell Nucleus
/ genetics
Chlamydomonas reinhardtii
/ genetics
Cyclin-Dependent Kinases
/ genetics
DNA Replication
/ genetics
Minichromosome Maintenance Complex Component 4
/ genetics
Minichromosome Maintenance Complex Component 6
/ genetics
Mitosis
/ genetics
Mutation
/ genetics
Origin Recognition Complex
/ genetics
Phosphorylation
/ genetics
Plasmids
/ genetics
Proteolysis
Ribonucleotide Reductases
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
04
08
2020
accepted:
07
03
2021
entrez:
28
4
2021
pubmed:
29
4
2021
medline:
26
8
2021
Statut:
epublish
Résumé
DNA replication is fundamental to all living organisms. In yeast and animals, it is triggered by an assembly of pre-replicative complex including ORC, CDC6 and MCMs. Cyclin Dependent Kinase (CDK) regulates both assembly and firing of the pre-replicative complex. We tested temperature-sensitive mutants blocking Chlamydomonas DNA replication. The mutants were partially or completely defective in DNA replication and did not produce mitotic spindles. After a long G1, wild type Chlamydomonas cells enter a division phase when it undergoes multiple rapid synchronous divisions ('multiple fission'). Using tagged transgenic strains, we found that MCM4 and MCM6 were localized to the nucleus throughout the entire multiple fission division cycle, except for transient cytoplasmic localization during each mitosis. Chlamydomonas CDC6 was transiently localized in nucleus in early division cycles. CDC6 protein levels were very low, probably due to proteasomal degradation. CDC6 levels were severely reduced by inactivation of CDKA1 (CDK1 ortholog) but not the plant-specific CDKB1. Proteasome inhibition did not detectably increase CDC6 levels in the cdka1 mutant, suggesting that CDKA1 might upregulate CDC6 at the transcriptional level. All of the DNA replication proteins tested were essentially undetectable until late G1. They accumulated specifically during multiple fission and then were degraded as cells completed their terminal divisions. We speculate that loading of origins with the MCM helicase may not occur until the end of the long G1, unlike in the budding yeast system. We also developed a simple assay for salt-resistant chromatin binding of MCM4, and found that tight MCM4 loading was dependent on ORC1, CDC6 and MCM6, but not on RNR1 or CDKB1. These results provide a microbial framework for approaching replication control in the plant kingdom.
Identifiants
pubmed: 33909603
doi: 10.1371/journal.pgen.1009471
pii: PGENETICS-D-20-01215
pmc: PMC8081180
doi:
Substances chimiques
Cell Cycle Proteins
0
Origin Recognition Complex
0
Saccharomyces cerevisiae Proteins
0
Ribonucleotide Reductases
EC 1.17.4.-
Rnr1 protein, S cerevisiae
EC 1.17.4.-
Cyclin-Dependent Kinases
EC 2.7.11.22
MCM4 protein, S cerevisiae
EC 3.6.4.12
MCM6 protein, S cerevisiae
EC 3.6.4.12
Minichromosome Maintenance Complex Component 4
EC 3.6.4.12
Minichromosome Maintenance Complex Component 6
EC 3.6.4.12
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009471Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM078153
Pays : United States
Organisme : NIGMS NIH HHS
ID : SC1 GM121242
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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