Assessing the potential use of chitosan scaffolds for the sustained localized delivery of vitamin D.
Chitosan
Dental pulp mesenchymal stem cells
Scaffold
vitamin D
Journal
Saudi journal of biological sciences
ISSN: 1319-562X
Titre abrégé: Saudi J Biol Sci
Pays: Saudi Arabia
ID NLM: 101543796
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
24
11
2020
revised:
04
12
2020
accepted:
06
01
2021
entrez:
29
4
2021
pubmed:
30
4
2021
medline:
30
4
2021
Statut:
ppublish
Résumé
Vitamin D is a commonly used bone modulator in regenerative medicine. Several modalities have been explored for the delivery of vitamin D including nanoparticles and scaffold. The present study aimed to assess the potential use of a bio-degradable chitosan scaffold for the delivery of vitamin D. The objectives included fabrication of a bio-degradable chitosan scaffold, integration of vitamin D into the scaffold, characterization of the vitamin D integrated scaffold. Characterization was carried out using, X-ray diffraction, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The structure of the scaffold was assessed by scanning electron microscopy. The scaffold was placed in phosphate buffer saline and the release duration of vitamin D was observed using UV spectrophotometry. Dental pulp mesenchymal stem cells were added to the scaffold to study the scaffold associated toxicity and the functionality of the scaffold released vitamin D. The vitamin D release period from the scaffold was estimated to be for 80 hrs. MTT assay of the stem cells was comparable to that of the control group (stem cells cultured in media) inferring that the scaffold is not toxic towards the stem cells. The positive alizarin red S staining, a higher expression of alkaline phosphatase, osteocalcin, and RunX2 confirmed the functional capability (osteogenic differentiation of the stem cells) of the released vitamin D. Based on the data from the present study, it can be inferred that chitosan scaffold can be used for the sustained delivery of functional vitamin D for 3-5 days.
Identifiants
pubmed: 33911937
doi: 10.1016/j.sjbs.2021.01.008
pii: S1319-562X(21)00007-3
pmc: PMC8071810
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2210-2215Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The results of the study have been filed as a patent (Application number – 202021041322)
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