Separation and anti-inflammatory evaluation of phytochemical constituents from Pleurospermum candollei (Apiaceae) by high-speed countercurrent chromatography with continuous sample load.
Animals
Anti-Inflammatory Agents
/ isolation & purification
Apiaceae
/ chemistry
Countercurrent Distribution
/ classification
Furocoumarins
/ isolation & purification
Macrophages
/ drug effects
Mice
Nitric Oxide
/ antagonists & inhibitors
Phytochemicals
/ isolation & purification
Plant Extracts
/ chemistry
Pyrogallol
/ analogs & derivatives
RAW 264.7 Cells
Pleurospermum candollei
anti-inflammatory
asarone
furanocoumarins
high-speed countercurrent chromatography
isoelemicin
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
revised:
19
04
2021
received:
25
02
2021
accepted:
26
04
2021
pubmed:
30
4
2021
medline:
15
12
2021
entrez:
29
4
2021
Statut:
ppublish
Résumé
Pleurospermum (Apiaceae) species possess a wide range of biological properties viz. analgesic, anti-inflammatory, antimalarial, and so on. Pleurospermum candollei (DC.) Benth. Ex C. B. Clark. is reported to cure diarrhea, gastric, respiratory, stomach, abdominal, joint, and back pain problems. In addition, it is also used for both male and female infertility. The present study deals with an efficient technique using high-speed countercurrent chromatography for separation of chemical components from the methanol extract of P. candollei. Notably, nine main compounds namely luteolin 7-O-glucoside (1), oxypeucedanin hydrate (2), pabulenol (3), bergapten (4), heptadecanoic acid (5), (E)-isoelemicin (6), trans-asarone (7), α-linolenic acid (8), and isoimperatorin (9) were very efficiently separated and isolated in pure form. Multiple injections were applied followed by two off-line recycling high-speed countercurrent chromatography. The inhibitory effect of nitric oxide production of all compounds was tested in the presence of 200 ng/mL lipopolysaccharide in RAW264.7 mice macrophage cells. The results demonstrated that compounds 7 and 8 effectively inhibited nitric oxide production, with IC
Identifiants
pubmed: 33914393
doi: 10.1002/jssc.202100155
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Furocoumarins
0
Phytochemicals
0
Plant Extracts
0
Pyrogallol
01Y4A2QXY0
Nitric Oxide
31C4KY9ESH
isoelemicin
487-12-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2663-2673Subventions
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2020MH396
Organisme : Science, Education and Industry Integration Innovation Pilot Project from Qilu University of Technology (Shandong Academy of Sciences)
ID : 2020KJC-GH08
Informations de copyright
© 2021 Wiley-VCH GmbH.
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