Caveolae-Mediated Endocytosis Drives Robust siRNA Delivery of Polymeric Nanoparticles to Macrophages.

Animals Caveolae Endocytosis Macrophages / metabolism Mice Nanoparticles RNA Interference RNA, Small Interfering / genetics Tumor Necrosis Factor-alpha / metabolism
CvME NPC1 Syntaxin6 anti-inflammatory efficacy cytosolic delivery key organelles siRNA drugs

Journal

ACS nano
ISSN: 1936-086X
Titre abrégé: ACS Nano
Pays: United States
ID NLM: 101313589

Informations de publication

Date de publication:
25 05 2021
Historique:
pubmed: 30 4 2021
medline: 10 6 2021
entrez: 29 4 2021
Statut: ppublish

Résumé

Cytosolic delivery of small interfering RNA (siRNA) remains challenging, and a profound understanding of the cellular uptake and intracellular processing of siRNA delivery systems could greatly improve the development of siRNA-based therapeutics. Here, we show that caveolae-mediated endocytosis (CvME) accounts for the robust siRNA delivery of mannose-modified trimethyl chitosan-cysteine/tripolyphosphate nanoparticles (MTC/TPP NPs) to macrophages by circumventing lysosomes. We show that the Golgi complex and ER are key organelles required for the efficient delivery of siRNA to macrophages in which the siRNA accumulation positively correlates with its silencing efficiency (

Identifiants

DOI: 10.1021/acsnano.0c08596 PMID: 33915044
pubmed: 33915044
doi: 10.1021/acsnano.0c08596
doi:

Substances chimiques

RNA, Small Interfering 0
Tumor Necrosis Factor-alpha 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

8267-8282

Auteurs

Yudong Song (Y)

State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200438, P.R. China.

Yanhua Wu (Y)

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, P.R. China.

Lu Xu (L)

State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200438, P.R. China.

Ting Jiang (T)

State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200438, P.R. China.

Cui Tang (C)

State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200438, P.R. China.
ORCID: 0000-0002-3705-6599

Chunhua Yin (C)

State Key Laboratory of Genetic Engineering, Department of Pharmaceutical Sciences, School of Life Sciences, Fudan University, Shanghai 200438, P.R. China.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Caveolae-Mediated Endocytosis Drives Robust...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Vésicules de transport › Vésicules tapissées › Cavéoles › Caveolae-Mediated Endocytosis Drives Robust...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Endocytose › Caveolae-Mediated Endocytosis Drives Robust...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Caveolae-Mediated Endocytosis Drives Robust...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Caveolae-Mediated Endocytosis Drives Robust...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Caveolae-Mediated Endocytosis Drives Robust...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › Caveolae-Mediated Endocytosis Drives Robust...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › Petit ARN interférent › Caveolae-Mediated Endocytosis Drives Robust...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Facteurs de nécrose tumorale › Facteur de nécrose tumorale alpha › Caveolae-Mediated Endocytosis Drives Robust...