Improvement of the immunogenicity of ESAT-6 via fusion with the dodecameric protein dodecin of Mycobacterium tuberculosis.

Animals Antigens, Bacterial BCG Vaccine Bacterial Proteins / genetics Mice Mycobacterium bovis Mycobacterium tuberculosis Tuberculosis Vaccines
Dodecin ESAT-6 Immunogenicity Multimeric fusion protein Mycobacterium tuberculosis

Journal

Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191

Informations de publication

Date de publication:
Jun 2021
Historique:
received: 14 01 2021
revised: 21 03 2021
accepted: 22 03 2021
pubmed: 30 4 2021
medline: 22 6 2021
entrez: 29 4 2021
Statut: ppublish

Résumé

Tuberculosis (TB) is a chronic infectious disease that creates a heavy medical burden worldwide. The only approved vaccine, Bacillus Calmette-Guérin (BCG), cannot fully protect adolescents and adults from TB. Therefore, there is an urgent need to develop an effective new vaccine. Previous studies have found that dodecin, a flavin-binding protein of Mycobacterium tuberculosis (Mtb), can form stable dodecamers and has the potential to improve the immunogenicity of Mtb antigens. In this study, we constructed the fusion protein dodecin-ESAT-6 and evaluated the immunogenicity of dodecin, ESAT-6, and dodecin-ESAT-6 separately. Our results showed that dodecin-ESAT-6 is a dodecameric protein that can withstand heat at 95 °C and under SDS-PAGE conditions. Dodecin-ESAT-6 increased the expression of the costimulatory molecules CD80, CD86, and major histocompatibility complex class II (MHC-II) on the surface of RAW264.7 macrophages. Mice immunized with dodecin-ESAT-6 exhibited higher percentages of antigen-specific CD4

Identifiants

DOI: 10.1016/j.micpath.2021.104890 PMID: 33915207
pubmed: 33915207
pii: S0882-4010(21)00162-5
doi: 10.1016/j.micpath.2021.104890
pii:
doi:

Substances chimiques

Antigens, Bacterial 0
BCG Vaccine 0
Bacterial Proteins 0
Tuberculosis Vaccines 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

104890

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Rongchuan Zhao (R)

Laboratory of Infection and Immunity, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China. Electronic address: 986047019@qq.com.

Tao Luo (T)

Laboratory of Infection and Immunity, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China. Electronic address: taoluo@scu.edu.cn.

Pengjiao Ma (P)

Laboratory of Infection and Immunity, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China. Electronic address: 1099861518@qq.com.

Liang Ge (L)

Laboratory of Infection and Immunity, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China. Electronic address: geliang255@163.com.

Zonghai Chen (Z)

Laboratory of Infection and Immunity, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China. Electronic address: zonghaichen@163.com.

Xinyan Wang (X)

Laboratory of Infection and Immunity, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China. Electronic address: karorinewang@163.com.

Wei Liao (W)

Laboratory of Infection and Immunity, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China. Electronic address: 619241828@qq.com.

Lang Bao (L)

Laboratory of Infection and Immunity, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, China. Electronic address: baolang@scu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Improvement of the immunogenicity of ESAT-6...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes bactériens › Improvement of the immunogenicity of ESAT-6...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins antibactériens › Vaccins antituberculeux › Vaccin BCG › Improvement of the immunogenicity of ESAT-6...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Improvement of the immunogenicity of ESAT-6...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Improvement of the immunogenicity of ESAT-6...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Mycobacteriaceae › Mycobacterium › Mycobacterium bovis › Improvement of the immunogenicity of ESAT-6...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis › Improvement of the immunogenicity of ESAT-6...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins antibactériens › Vaccins antituberculeux › Improvement of the immunogenicity of ESAT-6...