X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples.
X-ray fluorescence
XFI
microsample
nanoparticles
uptake
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
01 Apr 2021
01 Apr 2021
Historique:
received:
18
03
2021
accepted:
30
03
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
18
5
2021
Statut:
epublish
Résumé
Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.
Identifiants
pubmed: 33916283
pii: ijms22073691
doi: 10.3390/ijms22073691
pmc: PMC8037401
pii:
doi:
Substances chimiques
Gold
7440-57-5
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : 390715994
Organisme : Bundesministerium für Bildung und Forschung
ID : 02NUK032
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