Combined Assessment of Immune Checkpoint Regulator VISTA on Tumor-Associated Immune Cells and Platelet-to-Lymphocyte Ratio Identifies Advanced Germ Cell Tumors with Higher Risk of Unfavorable Outcomes.

PD-L1 VISTA biomarker germ cell tumor immunohistochemistry inflammation prognosis testicular cancer tumor microenvironment

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
07 Apr 2021
Historique:
received: 28 01 2021
revised: 16 03 2021
accepted: 02 04 2021
entrez: 30 4 2021
pubmed: 1 5 2021
medline: 1 5 2021
Statut: epublish

Résumé

In the current study, we aimed to investigate whether expression of immune checkpoint proteins (V-domain Ig suppressor of T cell activation (VISTA) and programmed death-ligand 1 (PD-L1)) and markers of systemic inflammation could predict progression/relapse and death in the cohort of 180 patients with testicular germ-cell tumors (GCTs). Expression of PD-L1 and VISTA was assessed by immunohistochemistry utilizing tissue microarrays. To estimate systemic inflammation neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), and platelet-to-lymphocyte ratio (PLR) were calculated. We found high PD-L1 and VISTA expression on tumor-associated immune cells (TAICs) in 89 (49.44%) and 63 (37.22%) of GCTs, respectively, whereas tumor cells besides trophoblastic elements were almost uniformly negative. High PD-L1 was associated with seminomatous histology and lower stage. Relapses in stage I patients occurred predominantly in cases with low numbers of PD-L1 and VISTA-expressing TAICs. In stage II/III disease, the combination of low VISTA-expressing TAICs and high PLR was identified as predictor of shorter event-free survival (HR 4.10; 1.48-11.36,

Identifiants

pubmed: 33916925
pii: cancers13081750
doi: 10.3390/cancers13081750
pmc: PMC8067539
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Gdański Uniwersytet Medyczny
ID : 01-0395/08/643

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Auteurs

Rafał Pęksa (R)

Department of Pathomorphology, Medical University of Gdansk, 80214 Gdansk, Poland.

Michał Kunc (M)

Department of Pathomorphology, Medical University of Gdansk, 80214 Gdansk, Poland.

Marta Popęda (M)

Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology, Medical University of Gdansk, 80211 Gdansk, Poland.

Michał Piątek (M)

Department of Clinical Oncology/Chemotherapy, St Barbara Regional Specialist Hospital No 5, 41200 Sosnowiec, Poland.

Michał Bieńkowski (M)

Department of Pathomorphology, Medical University of Gdansk, 80214 Gdansk, Poland.

Jolanta Żok (J)

Department of Oncology, Regional Oncology Center in Gdansk, 80219 Gdansk, Poland.

Anna Starzyńska (A)

Department of Oral Surgery, Medical University of Gdansk, 80211 Gdansk, Poland.

Adrian Perdyan (A)

Student Scientific Circle of Pathomorphology, Medical University of Gdansk, 80214 Gdansk, Poland.

Marek Sowa (M)

Department of Urology, Medical University of Gdansk, 80214 Gdansk, Poland.

Renata Duchnowska (R)

Department of Oncology, Military Institute in Warsaw, 01755 Warsaw, Poland.

Wojciech Biernat (W)

Department of Pathomorphology, Medical University of Gdansk, 80214 Gdansk, Poland.

Classifications MeSH