Genotoxic Bystander Signals from Irradiated Human Mesenchymal Stromal Cells Mainly Localize in the 10-100 kDa Fraction of Conditioned Medium.
Aged
Aged, 80 and over
Antigens, CD34
/ metabolism
Bystander Effect
/ drug effects
Cell Proliferation
/ drug effects
Chromosomal Instability
/ drug effects
Culture Media, Conditioned
/ pharmacology
DNA Damage
Female
Humans
Male
Mesenchymal Stem Cells
/ drug effects
Middle Aged
Molecular Weight
Mutagens
/ toxicity
X-Rays
CD34+ cells
bystander signals
leukemia
mesenchymal stromal cells
radiation-induced bystander effects
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
07 04 2021
07 04 2021
Historique:
received:
23
03
2021
revised:
03
04
2021
accepted:
04
04
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
25
2
2023
Statut:
epublish
Résumé
Genotoxic bystander signals released from irradiated human mesenchymal stromal cells (MSC) may induce radiation-induced bystander effects (RIBEs) in human hematopoietic stem and progenitor cells (HSPC), potentially causing leukemic transformation. Although the source of bystander signals is evident, the identification and characterization of these signals is challenging. Here, RIBEs were analyzed in human CD34+ cells cultured in distinct molecular size fractions of medium, conditioned by 2 Gy irradiated human MSC. Specifically, γH2AX foci (as a marker of DNA double-strand breaks) and chromosomal instability were evaluated in CD34+ cells grown in approximate (I) < 10 kDa, (II) 10-100 kDa and (III) > 100 kDa fractions of MSC conditioned medium and un-/fractionated control medium, respectively. Hitherto, significantly increased numbers of γH2AX foci (
Identifiants
pubmed: 33916980
pii: cells10040827
doi: 10.3390/cells10040827
pmc: PMC8067571
pii:
doi:
Substances chimiques
Antigens, CD34
0
Culture Media, Conditioned
0
Mutagens
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Stefan Jentsch Fellowship, Deutsche Gesellschaft für DNA-Reparaturforschung (DGDR)
ID : Stefan Jentsch Fellowship 2020
Organisme : José Carreras Leukämie-Stiftung
ID : DJCLS 14 R/2017
Références
Oncogene. 2008 Jan 17;27(4):434-40
pubmed: 17621264
Carcinogenesis. 2006 Feb;27(2):245-51
pubmed: 16150894
Nature. 2015 Feb 26;518(7540):552-555
pubmed: 25487151
Radiat Res. 2006 Sep;166(3):479-87
pubmed: 16953666
Radiat Res. 2010 Jun;173(6):769-78
pubmed: 20518656
Radiat Res. 1996 Oct;146(4):369-73
pubmed: 8927708
Cancer Sci. 2018 Sep;109(9):2632-2640
pubmed: 29949679
Radiat Res. 1998 Mar;149(3):256-62
pubmed: 9496888
Nature. 1963 Mar 16;197:1080-1
pubmed: 13964326
Nature. 2017 Jul 27;547(7664):458-462
pubmed: 28723894
Cancer Res. 2003 Dec 1;63(23):8437-42
pubmed: 14679007
Cancer Res. 2007 Jun 15;67(12):5872-9
pubmed: 17575156
Br J Cancer. 2008 Jun 3;98(11):1839-44
pubmed: 18475304
Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2099-104
pubmed: 10681418
Cancer Res. 2007 May 1;67(9):4295-302
pubmed: 17483342
RNA Biol. 2015;12(12):1355-63
pubmed: 26488306
Radiat Res. 2006 Apr;165(4):400-9
pubmed: 16579652
Int J Radiat Biol. 2011 Jul;87(7):683-95
pubmed: 21294691
Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):473-8
pubmed: 11149936
Radiat Res. 2003 Sep;160(3):318-23
pubmed: 12926990
Int J Radiat Biol. 2013 Dec;89(12):1028-34
pubmed: 23786650
Cancer Res. 2005 Jul 1;65(13):5668-73
pubmed: 15994940
Cancer Res. 2000 Oct 15;60(20):5608-11
pubmed: 11059747
Radiat Prot Dosimetry. 2011 Feb;143(2-4):264-9
pubmed: 21183548
Cancer. 1972 Oct;30(4):889-94
pubmed: 4116908
J Clin Oncol. 2015 Nov 1;33(31):3641-9
pubmed: 26304885
Cancer Res. 1992 Nov 15;52(22):6394-6
pubmed: 1423287
PLoS One. 2018 Apr 5;13(4):e0195371
pubmed: 29621312
Cell Cycle. 2007 Sep 15;6(18):2210-2
pubmed: 17881892
Leuk Res. 2015 Mar;39(3):348-54
pubmed: 25573287
J Vis Exp. 2017 Nov 3;(129):
pubmed: 29155797
Sci Rep. 2019 Jun 24;9(1):9103
pubmed: 31235776
J Cell Commun Signal. 2019 Sep;13(3):343-356
pubmed: 30903603
Cancer Res. 2008 Apr 1;68(7):2233-40
pubmed: 18381429
Leuk Res. 2017 Jun;57:112-118
pubmed: 28359030
Leuk Res. 2017 Feb;53:20-27
pubmed: 27951415
Cancer Res. 2008 Oct 1;68(19):8122-6
pubmed: 18829571
Am J Hematol. 2015 May;90(5):E80-5
pubmed: 25653205