Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry.

DAB Diaminobenzidine-tetrahydrochloride-dihydrate gross section immunohistochemistry margin inking surgical margins tissue marking

Journal

Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052

Informations de publication

Date de publication:
08 04 2021
Historique:
received: 09 03 2021
revised: 01 04 2021
accepted: 06 04 2021
entrez: 30 4 2021
pubmed: 1 5 2021
medline: 21 10 2021
Statut: epublish

Résumé

Pathological biopsy protocols require tissue marking dye (TMD) for orientation. In some cases (e.g., close margin), additional immunohistochemical analyses can be necessary. Therefore, the correlation between the applied TMD during macroscopy and the examined TMD during microscopy is crucial for the correct orientation, the residual tumour status and the subsequent therapeutic regime. In this context, our group observed colour changes during routine immunohistochemistry. Tissue specimens were marked with various TMD and processed by two different methods. TMD (blue, red, black, yellow and green) obtained from three different providers (A, B and C, and Whiteout/Tipp-Ex

Identifiants

pubmed: 33917667
pii: cells10040835
doi: 10.3390/cells10040835
pmc: PMC8068012
pii:
doi:

Substances chimiques

Coloring Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Selina Kiefer (S)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.

Julia Huber (J)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.
Comprehensive Cancer Center Freiburg, Medical Center-University of Freiburg, 79106 Freiburg im Breisgau, Germany.

Hannah Füllgraf (H)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.

Kristin Sörensen (K)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.

Agnes Csanadi (A)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.

Maren Nicole Stillger (MN)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.
Faculty of Biology, University of Freiburg, 79106 Freiburg im Breisgau, Germany.
Institute of Molecular Medicine and Cell Research, Medical Center, University of Freiburg, 79106 Freiburg im Breisgau, Germany.

Martin Werner (M)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.
Comprehensive Cancer Center Freiburg, Medical Center-University of Freiburg, 79106 Freiburg im Breisgau, Germany.

Hans-Eckart Schaefer (HE)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.

Peter Bronsert (P)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.
Comprehensive Cancer Center Freiburg, Medical Center-University of Freiburg, 79106 Freiburg im Breisgau, Germany.

Konrad Aumann (K)

Institute for Surgical Pathology, Medical Center-University of Freiburg, Breisacherstr. 115A, 79106 Freiburg im Breisgau, Germany.
Center for Pathology Kempten Allgäu-Klinikverbund Allgäu, 87439 Kempten, Germany.

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Classifications MeSH