Investigation of the Differences in Antithrombin to Heparin Binding among Antithrombin Budapest 3, Basel, and Padua Mutations by Biochemical and In Silico Methods.
Antithrombin Basel
Antithrombin Budapest 3
Antithrombin Padua
antithrombin
heparin-binding site
molecular modelling
surface plasmon resonance
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
08 04 2021
08 04 2021
Historique:
received:
02
03
2021
revised:
31
03
2021
accepted:
06
04
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
18
9
2021
Statut:
epublish
Résumé
Antithrombin (AT) is a serine protease inhibitor, its activity is highly accelerated by heparin. Mutations at the heparin-binding region lead to functional defect, type II heparin-binding site (IIHBS) AT deficiency. The aim of this study was to investigate and compare the molecular background of AT Budapest 3 (p.Leu131Phe, ATBp3), AT Basel (p.Pro73Leu), and AT Padua (p.Arg79His) mutations. Advanced in silico methods and heparin-binding studies of recombinant AT proteins using surface plasmon resonance method were used. Crossed immunoelectrophoresis and Differential Scanning Fluorimetry (NanoDSF) were performed in plasma samples. Heparin affinity of AT Padua was the lowest (KD = 1.08 × 10
Identifiants
pubmed: 33917853
pii: biom11040544
doi: 10.3390/biom11040544
pmc: PMC8068293
pii:
doi:
Substances chimiques
Recombinant Proteins
0
antithrombin III Basel
0
antithrombin III Padua
0
Antithrombin III
9000-94-6
Heparin
9005-49-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Union under European Regional Development Fund
ID : GINOP-2.3.2-15-2016-00039
Organisme : National Research, Development and Innovation Office, Hungarian Ministry of Innovation and Technology
ID : OTKA K116228
Organisme : European Union under European Regional Development Fund
ID : GINOP-2.3.2–15-2016–00044
Organisme : European Union under European Regional Development Fund
ID : GINOP-2.3.3–15-2016–00020
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