Luminescent Phage-Based Detection of
cloning
diagnostics
feces
luminescent
nluc
phage
Journal
Pharmaceuticals (Basel, Switzerland)
ISSN: 1424-8247
Titre abrégé: Pharmaceuticals (Basel)
Pays: Switzerland
ID NLM: 101238453
Informations de publication
Date de publication:
09 Apr 2021
09 Apr 2021
Historique:
received:
08
03
2021
revised:
30
03
2021
accepted:
01
04
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
1
5
2021
Statut:
epublish
Résumé
Bacteriophages ("phages") infect and multiply within specific bacterial strains, causing lysis of their target. Due to the specific nature of these interactions, phages allow a high-precision approach for therapy which can also be exploited for the detection of phage-sensitive pathogens associated with chronic diseases due to gut microbiome imbalance. As rapid phage-mediated detection assays becoming standard-of-care diagnostic tools, they will advance the more widespread application of phage therapy in a precision approach. Using a conventional method and a new cloning approach to develop luminescent phages, we engineered two phages that specifically detect a disease-associated microbial strain. We performed phage sensitivity assays in liquid culture and in fecal matrices and tested the stability of spiked fecal samples stored under different conditions. Different reporter gene structures and genome insertion sites were required to successfully develop the two
Identifiants
pubmed: 33918942
pii: ph14040347
doi: 10.3390/ph14040347
pmc: PMC8069110
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
Crit Rev Environ Sci Technol. 2015 Sep 2;45(17):1827-1879
pubmed: 26246784
Sci Rep. 2015 Feb 10;5:8365
pubmed: 25666585
Biosecur Bioterror. 2010 Jun;8(2):155-69
pubmed: 20569057
Chem Rev. 2017 Oct 11;117(19):12415-12474
pubmed: 28953368
PLoS One. 2015 May 22;10(5):e0128036
pubmed: 26000737
J Microbiol Methods. 2013 Nov;95(2):156-61
pubmed: 23994352
J Comput Biol. 2012 May;19(5):455-77
pubmed: 22506599
Sensors (Basel). 2020 Mar 31;20(7):
pubmed: 32244369
Bacteriophage. 2016 Aug 11;6(3):e1220348
pubmed: 27738558
Appl Environ Microbiol. 1996 Apr;62(4):1133-40
pubmed: 8919773
N Engl J Med. 2001 Sep 13;345(11):784-9
pubmed: 11556297
ACS Synth Biol. 2017 Oct 20;6(10):1952-1961
pubmed: 28657724
Bioinformatics. 2016 Nov 15;32(22):3380-3387
pubmed: 27466620
Nat Rev Microbiol. 2018 Dec;16(12):760-773
pubmed: 30104690
ACS Synth Biol. 2013 Jun 21;2(6):327-36
pubmed: 23654261
Appl Microbiol Biotechnol. 2011 May;90(4):1333-45
pubmed: 21327407
Antibiotics (Basel). 2020 Nov 04;9(11):
pubmed: 33158063
Front Microbiol. 2020 Oct 23;11:594868
pubmed: 33193274
Methods Mol Biol. 2009;501:69-76
pubmed: 19066811
ACS Synth Biol. 2012 Sep 21;1(9):408-13
pubmed: 23651338
Microbiology (Reading). 2010 Nov;156(Pt 11):3216-3223
pubmed: 20705661
Nucleic Acids Res. 2020 Jan 8;48(D1):D606-D612
pubmed: 31667520
RSC Adv. 2020 May 14;10(31):18451-18468
pubmed: 35685616
Nat Commun. 2020 Oct 20;11(1):5294
pubmed: 33082347
Science. 2017 Oct 20;358(6361):359-365
pubmed: 29051379
Clin Pharmacol Ther. 2000 Sep;68(3):225-30
pubmed: 11014403
Environ Sci Pollut Res Int. 2017 Jan;24(1):42-51
pubmed: 26903133
Bacteriophage. 2011 Mar;1(2):101-110
pubmed: 22334866
Appl Environ Microbiol. 2020 May 19;86(11):
pubmed: 32245761
Front Microbiol. 2016 Jan 12;6:1543
pubmed: 26793178
Methods Mol Biol. 2019;1898:37-50
pubmed: 30570721
Cell Host Microbe. 2013 Aug 14;14(2):207-15
pubmed: 23954159
PLoS Biol. 2016 Aug 19;14(8):e1002533
pubmed: 27541692
Prim Care. 2018 Sep;45(3):467-484
pubmed: 30115335
Bacteriophage. 2012 Jul 1;2(3):159-167
pubmed: 23275867
Cell Syst. 2015 Sep 23;1(3):187-196
pubmed: 26973885
Sci Rep. 2016 Sep 14;6:33235
pubmed: 27624517
Appl Environ Microbiol. 1997 Aug;63(8):2961-5
pubmed: 9251182
ACS Chem Biol. 2012 Nov 16;7(11):1848-57
pubmed: 22894855
Folia Microbiol (Praha). 2014 Jan;59(1):53-61
pubmed: 23846555
Annu Rev Virol. 2018 Sep 29;5(1):453-476
pubmed: 30001182
J Clin Microbiol. 2009 Dec;47(12):3887-94
pubmed: 19828743
Proc Natl Acad Sci U S A. 2018 Jan 16;115(3):567-572
pubmed: 29298913
Microbiol Mol Biol Rev. 2016 Jun 15;80(3):629-61
pubmed: 27307579