Preclinical Application of Reduced Manipulated Processing Strategy to Collect Transplantable Hepatocytes: A Pilot and Feasibility Study.
cell processing
cell therapy
hepatocyte
reduced manipulated strategy
Journal
Journal of personalized medicine
ISSN: 2075-4426
Titre abrégé: J Pers Med
Pays: Switzerland
ID NLM: 101602269
Informations de publication
Date de publication:
21 Apr 2021
21 Apr 2021
Historique:
received:
16
03
2021
revised:
13
04
2021
accepted:
20
04
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
1
5
2021
Statut:
epublish
Résumé
The complex isolation and purification process of hepatocytes for transplantation is labor intensive and with great contamination risk. Here, as a pilot and feasibility study, we examined in vitro and in vivo hepatocyte isolation feasibility and cell function of Cell Saver Rat and pig liver cells were collected using this system and then cultured in vitro, and their hepatocyte-specific enzymes were characterized. We then transplanted the hepatocytes in an established acute liver-injured (retrorsine+D-galactosamine-treated) rat model for engraftment. Recipient rats were sacrificed 1, 2, and 4 weeks after transplantation, followed by donor-cell identification and histological, serologic, and immunohistopathological examination. To demonstrate this Cell Saver We noted that in situ collagenase perfusion was followed by filtration, centrifugation, and collection in the Cell Saver Cell Saver
Sections du résumé
BACKGROUND
BACKGROUND
The complex isolation and purification process of hepatocytes for transplantation is labor intensive and with great contamination risk. Here, as a pilot and feasibility study, we examined in vitro and in vivo hepatocyte isolation feasibility and cell function of Cell Saver
METHODS
METHODS
Rat and pig liver cells were collected using this system and then cultured in vitro, and their hepatocyte-specific enzymes were characterized. We then transplanted the hepatocytes in an established acute liver-injured (retrorsine+D-galactosamine-treated) rat model for engraftment. Recipient rats were sacrificed 1, 2, and 4 weeks after transplantation, followed by donor-cell identification and histological, serologic, and immunohistopathological examination. To demonstrate this Cell Saver
RESULTS
RESULTS
We noted that in situ collagenase perfusion was followed by filtration, centrifugation, and collection in the Cell Saver
CONCLUSIONS
CONCLUSIONS
Cell Saver
Identifiants
pubmed: 33919203
pii: jpm11050326
doi: 10.3390/jpm11050326
pmc: PMC8143084
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Taiwan University Hospital
ID : 106-S3431
Organisme : National Taiwan University Hospital
ID : 108-S4162
Références
Liver Transpl. 2015 May;21(5):652-61
pubmed: 25821041
Sci Rep. 2017 Feb 02;7:41872
pubmed: 28150746
Nature. 2017 Jun 22;546(7659):533-538
pubmed: 28614297
EMBO Rep. 2017 Sep;18(9):1490-1492
pubmed: 28743713
J Hepatol. 2015 Apr;62(1 Suppl):S157-69
pubmed: 25920085
Cytotherapy. 2017 Dec;19(12):1400-1411
pubmed: 28964743
Liver Transpl. 2009 Jan;15(1):7-14
pubmed: 19109838
Nat Med. 2017 Aug;23(8):954-963
pubmed: 28671689
Hepatology. 2007 Nov;46(5):1464-75
pubmed: 17929236
Cell Stem Cell. 2016 Jun 2;18(6):797-808
pubmed: 26923201
Nat Med. 2019 Oct;25(10):1560-1565
pubmed: 31591593
Liver Int. 2016 May;36(5):761-2
pubmed: 26714614
Gut. 2019 Dec;68(12):2228-2237
pubmed: 31300517
Cell Transplant. 2009;18(10):1081-92
pubmed: 19650970
Asian J Surg. 2012 Jan;35(1):9-15
pubmed: 22726558
PLoS One. 2015 Sep 25;10(9):e0138655
pubmed: 26407160
ASAIO J. 1995 Apr-Jun;41(2):155-61
pubmed: 7640419
Mayo Clin Proc. 2014 Mar;89(3):414-24
pubmed: 24582199
J Hepatol. 2010 Oct;53(4):769-74
pubmed: 20667616
Semin Liver Dis. 2019 Nov;39(4):442-451
pubmed: 31242527
Hepatology. 1992 Jun;15(6):1048-53
pubmed: 1350563
Cell Transplant. 2015;24(12):2557-71
pubmed: 25706818
J Mol Med (Berl). 2018 Jun;96(6):469-481
pubmed: 29691598
Expert Rev Gastroenterol Hepatol. 2020 Mar;14(3):185-196
pubmed: 32098516
Circ Res. 2007 May 11;100(9):1249-60
pubmed: 17495232
J Cell Biol. 1964 Feb;20:271-9
pubmed: 14126872
Hepatology. 1996 Mar;23(3):482-96
pubmed: 8617428
PLoS One. 2015 Aug 26;10(8):e0134327
pubmed: 26308208
World J Gastroenterol. 2016 Jan 14;22(2):874-86
pubmed: 26811633