Longitudinal Analysis and Comparison of Six Serological Assays up to Eight Months Post-COVID-19 Diagnosis.

COVID-19 SARS-CoV-2 assay performance serological assays

Journal

Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588

Informations de publication

Date de publication:
21 Apr 2021
Historique:
received: 26 02 2021
revised: 09 04 2021
accepted: 20 04 2021
entrez: 30 4 2021
pubmed: 1 5 2021
medline: 1 5 2021
Statut: epublish

Résumé

There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce. We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight months after diagnosis by RT-PCR using several commercial assays. Among the 208 samples tested, the percentage of seropositivity was comparable between assays up to four months after diagnosis and then tended to be more heterogeneous between assays ( In conclusion, serological tests show equivalent sensitivity in the first months after the diagnosis of SARS-CoV-2 infection, but their performance later, postinfection, must be considered when interpreting the results.

Sections du résumé

BACKGROUND BACKGROUND
There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce.
METHODS METHODS
We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight months after diagnosis by RT-PCR using several commercial assays.
RESULTS RESULTS
Among the 208 samples tested, the percentage of seropositivity was comparable between assays up to four months after diagnosis and then tended to be more heterogeneous between assays (
CONCLUSIONS CONCLUSIONS
In conclusion, serological tests show equivalent sensitivity in the first months after the diagnosis of SARS-CoV-2 infection, but their performance later, postinfection, must be considered when interpreting the results.

Identifiants

pubmed: 33919328
pii: jcm10091815
doi: 10.3390/jcm10091815
pmc: PMC8122444
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Aurélien Aubry (A)

Department of Virology, Amiens University Medical Center, 80000 Amiens, France.
Resistant Infectious Agents and Chemotherapy Research Unit, AGIR UR4294, Jules Verne University of Picardie, 80000 Amiens, France.

Baptiste Demey (B)

Department of Virology, Amiens University Medical Center, 80000 Amiens, France.
Resistant Infectious Agents and Chemotherapy Research Unit, AGIR UR4294, Jules Verne University of Picardie, 80000 Amiens, France.

Catherine François (C)

Department of Virology, Amiens University Medical Center, 80000 Amiens, France.
Resistant Infectious Agents and Chemotherapy Research Unit, AGIR UR4294, Jules Verne University of Picardie, 80000 Amiens, France.

Gilles Duverlie (G)

Department of Virology, Amiens University Medical Center, 80000 Amiens, France.
Resistant Infectious Agents and Chemotherapy Research Unit, AGIR UR4294, Jules Verne University of Picardie, 80000 Amiens, France.

Sandrine Castelain (S)

Department of Virology, Amiens University Medical Center, 80000 Amiens, France.
Resistant Infectious Agents and Chemotherapy Research Unit, AGIR UR4294, Jules Verne University of Picardie, 80000 Amiens, France.

François Helle (F)

Department of Virology, Amiens University Medical Center, 80000 Amiens, France.
Resistant Infectious Agents and Chemotherapy Research Unit, AGIR UR4294, Jules Verne University of Picardie, 80000 Amiens, France.

Etienne Brochot (E)

Department of Virology, Amiens University Medical Center, 80000 Amiens, France.
Resistant Infectious Agents and Chemotherapy Research Unit, AGIR UR4294, Jules Verne University of Picardie, 80000 Amiens, France.

Classifications MeSH