Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Can Extensively Differentiate to Tyrosine-Hydroxylase-Expressing Dopamine-Secreting Neurons.
differentiation
dopamine
hair follicle
hair follicle stem cell area
neural stem cells
neurogenesis
neuron
stem cell
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
10 04 2021
10 04 2021
Historique:
received:
09
03
2021
revised:
08
04
2021
accepted:
08
04
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
21
10
2021
Statut:
epublish
Résumé
Hair-follicle-associated pluripotent (HAP) stem cells are located in the bulge area of hair follicles from mice and humans and have been shown to differentiate to neurons, glia, keratinocytes, smooth muscle cells, melanocytes and beating cardiac muscle cells in vitro. Subsequently, we demonstrated that HAP stem cells could effect nerve and spinal-cord regeneration in mouse models, differentiating to Schwann cells and neurons in this process. HAP stem cells can be banked by cryopreservation and preserve their ability to differentiate. In the present study, we demonstrated that mouse HAP stem cells cultured in neural-induction medium can extensively differentiate to dopaminergic neurons, which express tyrosine hydroxylase and secrete dopamine. These results indicate that the dopaminergic neurons differentiated from HAP stem cells may be useful in the future to improve the symptoms of Parkinson's disease in the clinic.
Identifiants
pubmed: 33920157
pii: cells10040864
doi: 10.3390/cells10040864
pmc: PMC8069047
pii:
doi:
Substances chimiques
Adenosine Triphosphate
8L70Q75FXE
Tyrosine 3-Monooxygenase
EC 1.14.16.2
Calcium
SY7Q814VUP
Dopamine
VTD58H1Z2X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Education and Science
ID : 19K08781
Organisme : Terumo Foundation for Life Sciences and Arts
ID : to Y. Amoh
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