Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection.


Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
17 04 2021
Historique:
received: 23 03 2021
revised: 09 04 2021
accepted: 15 04 2021
entrez: 30 4 2021
pubmed: 1 5 2021
medline: 19 8 2021
Statut: epublish

Résumé

This study reports on a fatal case of a captive great grey owl infected with the West Nile virus (WNV) in the zoological garden Košice, eastern Slovakia (Central Europe). The tissue samples of the dead owl were used for virus isolation and genetic characterization. The novel isolate is genetically closer to Hungarian, Greek, and Bulgarian strains from the central/southern European clade of lineage 2 than to the strains previously isolated in Slovakia. Interestingly, it carries NS3-249P, a molecular virulence determinant associated with higher neurovirulence, which has not previously been observed in Slovakia. Subsequent serological investigation of the captive owls revealed additional seropositive animals, indicating local WNV transmission. Although no WNV-positive mosquitoes were found, the presence of the WNV principal vector

Identifiants

pubmed: 33920598
pii: v13040699
doi: 10.3390/v13040699
pmc: PMC8073349
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Katarína Peňazziová (K)

Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia.

Ľuboš Korytár (Ľ)

Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia.

Patrik Pastorek (P)

ZOO Košice, Ulica k Zoologickej Záhrade 1, 04001 Košice, Slovakia.

Juraj Pistl (J)

Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia.

Diana Rusňáková (D)

Department of Molecular Biology, Faculty of Natural Sciences and Science Park, Comenius University in Bratislava, Ilkovičova 8, 84104 Bratislava, Slovakia.

Tomáš Szemes (T)

Department of Molecular Biology, Faculty of Natural Sciences and Science Park, Comenius University in Bratislava, Ilkovičova 8, 84104 Bratislava, Slovakia.

Viktória Čabanová (V)

Biomedical Research Center of Slovak Academy of Sciences, Department of Virus Ecology, Institute of Virology, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.

Martina Ličková (M)

Biomedical Research Center of Slovak Academy of Sciences, Department of Virus Ecology, Institute of Virology, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.

Kristína Boršová (K)

Biomedical Research Center of Slovak Academy of Sciences, Department of Virus Ecology, Institute of Virology, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 84215 Bratislava, Slovakia.

Boris Klempa (B)

Biomedical Research Center of Slovak Academy of Sciences, Department of Virus Ecology, Institute of Virology, Dúbravská Cesta 9, 84505 Bratislava, Slovakia.

Tomáš Csank (T)

Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, Slovakia.

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Classifications MeSH