Improved Detection of Intestinal Helminth Infections with a Formalin Ethyl-Acetate-Based Concentration Technique Compared to a Crude Formalin Concentration Technique.

diagnosis formalin ethyl-acetate concentration technique small liver flukes soil-transmitted helminths stool concentration technique

Journal

Tropical medicine and infectious disease
ISSN: 2414-6366
Titre abrégé: Trop Med Infect Dis
Pays: Switzerland
ID NLM: 101709042

Informations de publication

Date de publication:
15 Apr 2021
Historique:
received: 26 03 2021
revised: 12 04 2021
accepted: 13 04 2021
entrez: 30 4 2021
pubmed: 1 5 2021
medline: 1 5 2021
Statut: epublish

Résumé

Intestinal helminth infections are the most prevalent neglected tropical diseases, predominantly affecting rural and marginalised populations. The mainstay of diagnosis is the microscopic examination of faecal samples to detect parasites in the form of eggs, larvae and cysts. In an effort to improve the standard of care, the comparative accuracy in detecting helminth infections of the hitherto used formalin-based concentration method (FC) was compared to a previously developed formalin ethyl-acetate-based concentration technique (FECT), prior to the systematic deployment of the latter at a research and humanitarian unit operating on the Thailand-Myanmar border. A total of 693 faecal samples were available for the comparison of the two diagnostic methods. The FECT was superior in detecting hookworm,

Identifiants

pubmed: 33921041
pii: tropicalmed6020051
doi: 10.3390/tropicalmed6020051
pmc: PMC8167623
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Tobias Brummaier (T)

Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, P.O. Box 46, 68/30 Bann Thung Road, Mae Sot 63100, Thailand.
Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland.
University of Basel, P.O. Box, CH-4003 Basel, Switzerland.
Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7LG, UK.

Laypaw Archasuksan (L)

Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, P.O. Box 46, 68/30 Bann Thung Road, Mae Sot 63100, Thailand.

Dorn Watthanakulpanich (D)

Faculty of Tropical Medicine, Department of Helminthology, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand.

Daniel H Paris (DH)

Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland.
University of Basel, P.O. Box, CH-4003 Basel, Switzerland.

Jürg Utzinger (J)

Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland.
University of Basel, P.O. Box, CH-4003 Basel, Switzerland.

Rose McGready (R)

Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, P.O. Box 46, 68/30 Bann Thung Road, Mae Sot 63100, Thailand.
Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7LG, UK.

Stephane Proux (S)

Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, P.O. Box 46, 68/30 Bann Thung Road, Mae Sot 63100, Thailand.

François Nosten (F)

Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, P.O. Box 46, 68/30 Bann Thung Road, Mae Sot 63100, Thailand.
Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7LG, UK.

Classifications MeSH