Detection of Antimicrobial Peptides in Stratum Corneum by Mass Spectrometry.
antimicrobial peptide
healthy skin
mass spectrometry
stratum corneum
tape stripping
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
19 Apr 2021
19 Apr 2021
Historique:
received:
17
03
2021
revised:
13
04
2021
accepted:
15
04
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
25
5
2021
Statut:
epublish
Résumé
Antimicrobial and immunomodulatory peptides (AMPs) are considered as the key players in the maintenance of skin barrier functions. Here, we developed a novel approach for the examination of AMPs in the outermost layer of the epidermis, namely stratum corneum (SC). The SC sample collection by tape stripping was coupled with detection by highly specific and sensitive parallel reaction monitoring (PRM)-based mass spectrometry. We found that hexane-free processing of SC samples produced higher protein yield compared to hexane-based extraction. Of the 18 investigated peptides, 9 could be detected either in healthy or in inflamed skin specimens. Regarding the amount of S100A8, LCN2, LACRT and LYZ significant topographical differences were described among gland poor (GP), sebaceous gland rich (SGR) and apocrine gland rich (AGR) healthy skin regions. We applied a minimally invasive, reproducible approach for sampling, which can be assessed for research and diagnostic purposes and for monitoring the effectiveness of therapies in skin diseases.
Identifiants
pubmed: 33921817
pii: ijms22084233
doi: 10.3390/ijms22084233
pmc: PMC8073585
pii:
doi:
Substances chimiques
Pore Forming Cytotoxic Proteins
0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Hungarian Research Grants
ID : NKFIH K-128250
Organisme : EFOP
ID : 3.6.1-16-2016-00022
Organisme : GINOP
ID : 2.3.3-15-2016-00020
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