Plasmids Expressing shRNAs Specific to the Nucleocapsid Gene Inhibit the Replication of Porcine Deltacoronavirus In Vivo.
RNA interference
nucleocapsid
porcine deltacoronavirus
short hairpin RNA
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
23 Apr 2021
23 Apr 2021
Historique:
received:
17
03
2021
revised:
20
04
2021
accepted:
21
04
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
1
5
2021
Statut:
epublish
Résumé
Porcine deltacoronavirus (PDCoV) is a novel enteric coronavirus and is becoming one of the major causative agents of diarrhea in pig herds in recent years. To date, there are no commercial vaccines or antiviral pharmaceutical agents available to control PDCoV infection. Therefore, developing a reliable strategy against PDCoV is urgently needed. In this study, to observe the antiviral activity of RNA interference (RNAi), four short hairpin RNAs (shRNAs) specific to the nucleocapsid (N) gene of PDCoV were designed and tested in vitro. Of these, a double-shRNA-expression vector, designated as pSil-double-shRNA-N1, was the most effectively expressed, and the inhibition of PDCoV replication was then further evaluated in neonatal piglets. Our preliminary results reveal that plasmid-based double-shRNA-expression targeting the N gene of PDCoV can significantly protect LLC-PK1 cells and piglets from pathological lesions induced by PDCoV. Our study could benefit the investigation of the specific functions of viral genes related to PDCoV infection and offer a possible methodology of RNAi-based therapeutics for PDCoV infection.
Identifiants
pubmed: 33922444
pii: ani11051216
doi: 10.3390/ani11051216
pmc: PMC8145914
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Key Research and Development Program
ID : 2017YFD0500600
Organisme : National Natural Science Foundation of China
ID : 32002289
Organisme : Natural Science Foundation of Jiangxi Province
ID : 2018ACB21027
Organisme : Projects in Jiangxi Province
ID : YC2018-S200
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