Oxidative Stress and Alterations of Paraoxonases in Atopic Dermatitis.

atopic dermatitis myeloperoxidase oxidative stress paraoxonases

Journal

Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981

Informations de publication

Date de publication:
28 Apr 2021
Historique:
received: 07 04 2021
revised: 26 04 2021
accepted: 26 04 2021
entrez: 30 4 2021
pubmed: 1 5 2021
medline: 1 5 2021
Statut: epublish

Résumé

previous studies reported the involvement of reactive oxygen species (ROS) and lipid peroxidation in the pathogenesis of inflammatory skin diseases. The aim of our study was to investigate the relationship between oxidative stress and inflammation in children affected by atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. levels of lipid hydroperoxides, total antioxidant capacity, and activities of the enzymes myeloperoxidase (MPO), PON1, and PON2/3 were investigated in 56 atopic pediatric patients, and compared with 48 sex-/age-matched healthy controls. significantly higher levels of lipid hydroperoxides and lower values of total antioxidant potential were observed in the serum of AD children compared to that of the controls. Significant lower PON1 activities, and a significant increase in levels of MPO were observed in serum of patients, with a higher serum MPO level/PON1 paraoxonase activity ratio in patients compared to that in the controls. Significantly lower lactonase activity of PON enzymes was observed in polymorphonuclear cells isolated from AD patients. Statistically negative correlation was established between the activity of intracellular PON2/3 activity and ROS levels. our data confirmed that AD is associated with higher oxidative damage and a decrease in antioxidant defense. Moreover, alterations of extracellular and intracellular PON activity can promote lipoprotein dysfunction in AD patients.

Sections du résumé

BACKGROUND BACKGROUND
previous studies reported the involvement of reactive oxygen species (ROS) and lipid peroxidation in the pathogenesis of inflammatory skin diseases. The aim of our study was to investigate the relationship between oxidative stress and inflammation in children affected by atopic dermatitis (AD), a chronic relapsing inflammatory skin disease.
METHODS METHODS
levels of lipid hydroperoxides, total antioxidant capacity, and activities of the enzymes myeloperoxidase (MPO), PON1, and PON2/3 were investigated in 56 atopic pediatric patients, and compared with 48 sex-/age-matched healthy controls.
RESULTS RESULTS
significantly higher levels of lipid hydroperoxides and lower values of total antioxidant potential were observed in the serum of AD children compared to that of the controls. Significant lower PON1 activities, and a significant increase in levels of MPO were observed in serum of patients, with a higher serum MPO level/PON1 paraoxonase activity ratio in patients compared to that in the controls. Significantly lower lactonase activity of PON enzymes was observed in polymorphonuclear cells isolated from AD patients. Statistically negative correlation was established between the activity of intracellular PON2/3 activity and ROS levels.
CONCLUSIONS CONCLUSIONS
our data confirmed that AD is associated with higher oxidative damage and a decrease in antioxidant defense. Moreover, alterations of extracellular and intracellular PON activity can promote lipoprotein dysfunction in AD patients.

Identifiants

pubmed: 33925093
pii: antiox10050697
doi: 10.3390/antiox10050697
pmc: PMC8144960
pii:
doi:

Types de publication

Journal Article

Langues

eng

Références

J Biol Chem. 2001 Nov 30;276(48):44444-9
pubmed: 11579088
Curr Med Chem. 2020 Jul 15;:
pubmed: 32674726
Acta Derm Venereol Suppl (Stockh). 1994;186:38-40
pubmed: 8073834
Curr Drug Targets Inflamm Allergy. 2005 Aug;4(4):517-9
pubmed: 16127829
Ital J Pediatr. 2017 Feb 28;43(1):22
pubmed: 28245844
Bull Exp Biol Med. 2011 Apr;150(6):690-2
pubmed: 22235418
Dermatology. 2012;224(4):323-30
pubmed: 22710427
Antioxidants (Basel). 2020 Feb 26;9(3):
pubmed: 32111015
Ann Allergy Asthma Immunol. 2020 Jan;124(1):70-78
pubmed: 31622668
Br J Dermatol. 1998 Jun;138(6):1033-5
pubmed: 9747368
Acta Derm Venereol. 2009;89(2):122-5
pubmed: 19325993
Circulation. 2007 Apr 17;115(15):2055-64
pubmed: 17404154
Dermatology. 1993;186(1):23-31
pubmed: 8435513
Exp Ther Med. 2016 Dec;12(6):3863-3872
pubmed: 28105118
J Eur Acad Dermatol Venereol. 2009 Dec;23(12):1405-8
pubmed: 20205355
Br J Dermatol. 2012 Jan;166(1):204-7
pubmed: 21790517
J Clin Endocrinol Metab. 2005 Mar;90(3):1728-33
pubmed: 15613429
Front Pediatr. 2021 Jan 12;8:577413
pubmed: 33585361
J Clin Endocrinol Metab. 2004 Jun;89(6):2957-62
pubmed: 15181084
Antioxid Redox Signal. 2021 Jan 20;34(3):191-200
pubmed: 31969002
Arch Dermatol Res. 1986;278(6):454-9
pubmed: 3789804
Oxid Med Cell Longev. 2016;2016:2721469
pubmed: 27006746
Front Physiol. 2020 Jul 02;11:694
pubmed: 32714204
Biofactors. 2015 May 6;41(3):153-9
pubmed: 26072738
Pediatr Allergy Immunol. 2013 Aug;24(5):476-86
pubmed: 23773154
Arch Dermatol Res. 2020 Jan;312(1):33-39
pubmed: 31531730
FASEB J. 2014 Apr;28(4):1769-79
pubmed: 24421402
J Agric Food Chem. 2001 Oct;49(10):4619-26
pubmed: 11599998
Life Sci. 2001 Jun 1;69(2):223-8
pubmed: 11441912
J Clin Invest. 1999 Jun;103(11):1547-60
pubmed: 10359564
Biochem Med Metab Biol. 1989 Apr;41(2):132-8
pubmed: 2719858
Dermatology. 1992;185(2):96-100
pubmed: 1421638
Nutrition. 2012 Nov-Dec;28(11-12):1157-64
pubmed: 22964087
J Clin Diagn Res. 2013 Dec;7(12):2683-5
pubmed: 24551611
Curr Opin Lipidol. 2005 Aug;16(4):393-9
pubmed: 15990587
Free Radic Res. 2003 Jan;37(1):77-83
pubmed: 12653220
J Clin Invest. 2004 Aug;114(4):529-41
pubmed: 15314690
Atherosclerosis. 2014 Jun;234(2):288-94
pubmed: 24704632
Pediatr Res. 1993 May;33(5):506-9
pubmed: 8511025
Br J Dermatol. 2013 May;168(5):984-9
pubmed: 23614561
Biomedicines. 2020 Dec 01;8(12):
pubmed: 33271807
Curr Med Chem. 2009;16(6):667-75
pubmed: 19199930
Metabolism. 2003 Dec;52(12):1601-5
pubmed: 14669163
Atherosclerosis. 2011 Jan;214(1):20-36
pubmed: 20934178
JAMA. 2001 Nov 7;286(17):2136-42
pubmed: 11694155
J Biol Chem. 2006 Oct 6;281(40):29491-500
pubmed: 16891303
Arterioscler Thromb Vasc Biol. 2003 Mar 1;23(3):468-74
pubmed: 12615656
Front Pediatr. 2020 Dec 22;8:583946
pubmed: 33415087
J Biol Chem. 2009 Nov 6;284(45):30825-35
pubmed: 19726691
Arch Dermatol Res. 2015 Sep;307(7):617-23
pubmed: 25967637
Biomolecules. 2019 Dec 03;9(12):
pubmed: 31816846
Antioxid Redox Signal. 2009 Nov;11(11):2899-937
pubmed: 19622015
Allergy. 2019 Feb;74(2):398-402
pubmed: 30267572

Auteurs

Oriana Simonetti (O)

Department of Clinical and Molecular Sciences-Dermatology, Polytechnic University of Marche, I-60126 Ancona, Italy.

Tiziana Bacchetti (T)

Department of Life and Environmental Sciences-Biochemistry, Polytechnic University of Marche, I-60126 Ancona, Italy.

Gianna Ferretti (G)

Research Center of Health Education and Health Promotion, Department of Clinical Experimental Science and Odontostomatology-Biochemistry, Polytechnic University of Marche, I-60126 Ancona, Italy.

Elisa Molinelli (E)

Department of Clinical and Molecular Sciences-Dermatology, Polytechnic University of Marche, I-60126 Ancona, Italy.

Giulio Rizzetto (G)

Department of Clinical and Molecular Sciences-Dermatology, Polytechnic University of Marche, I-60126 Ancona, Italy.

Luisa Bellachioma (L)

Department of Life and Environmental Sciences-Biochemistry, Polytechnic University of Marche, I-60126 Ancona, Italy.

Annamaria Offidani (A)

Department of Clinical and Molecular Sciences-Dermatology, Polytechnic University of Marche, I-60126 Ancona, Italy.

Classifications MeSH