Imaging translation dynamics in live embryos reveals spatial heterogeneities.

Animals Drosophila melanogaster / embryology Embryo, Nonmammalian / metabolism Kinetics Protein Biosynthesis RNA, Messenger / genetics Single Molecule Imaging / methods

Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
21 May 2021
Historique:
received: 29 04 2020
accepted: 13 04 2021
pubmed: 1 5 2021
medline: 5 6 2021
entrez: 30 4 2021
Statut: ppublish

Résumé

Much is known about the factors involved in the translation of messenger RNA (mRNA) into protein; however, this multistep process has not been imaged in living multicellular organisms. Here, we deploy the SunTag method to visualize and quantify the timing, location, and kinetics of the translation of single mRNAs in living

Identifiants

DOI: 10.1126/science.abc3483 PMID: 33927056
pubmed: 33927056
pii: science.abc3483
doi: 10.1126/science.abc3483
doi:

Substances chimiques

RNA, Messenger 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

840-844

Informations de copyright

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Auteurs

Jeremy Dufourt (J)

Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS-UMR 5535, Montpellier 34293 cedex 5, France. jeremy.dufourt@igmm.cnrs.fr mounia.lagha@igmm.cnrs.fr.
ORCID: 0000-0003-4043-7250

Maelle Bellec (M)

Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS-UMR 5535, Montpellier 34293 cedex 5, France.
ORCID: 0000-0002-9029-0042

Antonio Trullo (A)

Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS-UMR 5535, Montpellier 34293 cedex 5, France.
ORCID: 0000-0001-7560-3859

Matthieu Dejean (M)

Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS-UMR 5535, Montpellier 34293 cedex 5, France.
ORCID: 0000-0003-4175-1954

Sylvain De Rossi (S)

MRI, BioCampus Montpellier, CNRS, INSERM, University of Montpellier, Montpellier, France.

Cyril Favard (C)

Institut de Recherche en Infectiologie de Montpellier, CNRS UMR 9004, University of Montpellier, Montpellier 34293 cedex 5, France.
ORCID: 0000-0002-8304-2980

Mounia Lagha (M)

Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS-UMR 5535, Montpellier 34293 cedex 5, France. jeremy.dufourt@igmm.cnrs.fr mounia.lagha@igmm.cnrs.fr.
ORCID: 0000-0002-7082-1950

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Imaging translation dynamics in live embryos...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Drosophila melanogaster › Imaging translation dynamics in live embryos...
questionsmedicales.fr › Structures de l'embryon › Embryon non mammalien › Imaging translation dynamics in live embryos...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Imaging translation dynamics in live embryos...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Biosynthèse des protéines › Imaging translation dynamics in live embryos...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Imaging translation dynamics in live embryos...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Imagerie moléculaire › Imagerie de molécules uniques › Imaging translation dynamics in live embryos...