Human and Murine T-Helper Cell Recovery from Organs and Tissues.
CD4+ T cells
Enzymatic digestion
Lymphoid organs
Peripheral tissues
T cell isolation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
24
6
2021
Statut:
ppublish
Résumé
Isolation of cells from organs and tissues represents a challenge for many researchers. Cell yield, purity, and cell death are common problems associated with it, which greatly affect experimental results in terms of reproducibility and biological observations. Here, we describe state-of-the-art protocols of how to isolate CD4
Identifiants
pubmed: 33928539
doi: 10.1007/978-1-0716-1311-5_1
doi:
Substances chimiques
Biomarkers
0
Enzymes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-25Références
Harris ED Jr, Krane SM (1974) Collagenases (first of three parts). N Engl J Med 291:557–563
doi: 10.1056/NEJM197409122911105
Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635
doi: 10.1128/MMBR.62.3.597-635.1998
Bond MD, Van Wart HE (1984) Characterization of the individual collagenases from Clostridium histolyticum. Biochemistry 23:3085–3091
doi: 10.1021/bi00308a036
Hui AY, McCarty WJ, Masuda K, Firestein GS, Sah RL (2012) A systems biology approach to synovial joint lubrication in health, injury, and disease. Wiley Interdiscip Rev Syst Biol Med 4(1):15–37
doi: 10.1002/wsbm.157
Mowat AM, Agace WW (2014) Regional specialization within the intestinal immune system. Nat Rev Immunol 14:667–685
doi: 10.1038/nri3738
Engelhardt B, Vajkoczy P, Weller RO (2017) The movers and shapers in immune privilege of the CNS. Nat Immunol 18(2):123–131
doi: 10.1038/ni.3666
Oliveira DS, Dzinic S, Bonfil AI, Saliganan AD, Sheng S, Bonfil RD (2016) The mouse prostate: a basic anatomical and histological guideline. Bosn J Basic Med Sci 16:8–13
pubmed: 26773172
pmcid: 4765945
Cortesi F, Delfanti G, Grilli A, Calcinotto A, Gorini F, Pucci F, Luciano R, Grioni M, Recchia A, Benigni F et al (2018) Bimodal CD40/Fas-dependent crosstalk between iNKT cells and tumor-associated macrophages impairs prostate cancer progression. Cell Rep 22:3006–3020
doi: 10.1016/j.celrep.2018.02.058
Kohlgruber AC, Gal-Oz ST, LaMarche NM, Shimazaki M, Duquette D, Koay HF, Nguyen HN, Mina AI, Paras T, Tavakkoli A et al (2018) Gammadelta T cells producing interleukin-17A regulate adipose regulatory T cell homeostasis and thermogenesis. Nat Immunol 19:464–474
doi: 10.1038/s41590-018-0094-2
Benner R, van Oudenaaren A, Koch G (1981) Induction of antibody formation in mouse bone marrow. In: Lefkovits I, Pernis B (eds) Immunological methods, vol II. Academic Press, New York, pp 247–261
doi: 10.1016/B978-0-12-442702-0.50020-0
Anderson KG, Mayer-Barber K, Sung H, Beura L, James BR, Taylor JJ, Qunaj L, Griffith TS, Vezys V, Barber DL, Masopust D (2014) Intravascular staining for discrimination of vascular and tissue leukocytes. Nat Protoc 9:209–222
doi: 10.1038/nprot.2014.005