Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation.
Cytokines
Flow cytometry
Intracellular staining
PMA/ionomycin
T cell activation
Th cells
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
24
6
2021
Statut:
ppublish
Résumé
An important hallmark for the characterisation of Th cells is their capacity for cytokine expression. In this chapter, we describe how Th cells can be restimulated polyclonally to reveal their cytokine-producing potential that can then be analysed by intracellular staining and flow cytometry.
Identifiants
pubmed: 33928547
doi: 10.1007/978-1-0716-1311-5_9
doi:
Substances chimiques
Cytokines
0
Ionomycin
56092-81-0
Tetradecanoylphorbol Acetate
NI40JAQ945
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111-119Références
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