mRNA Transfection of T-Lymphocytes by Electroporation.
CCR5-knockout
CD4+-T cells isolation
Electroporation
Gene editing
Gene therapy
mRNA transfection
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
24
6
2021
Statut:
ppublish
Résumé
Electroporation enables the transfection of different cell types including microbial, plant, and animal cells with charged molecules, such as nuclear acids or proteins. During electroporation, an electrical field is applied to the cells leading to a transient permeabilization of the cell membrane allowing exogenous molecules to enter the cells. Here we report the electroporation of human primary CD4
Identifiants
pubmed: 33928556
doi: 10.1007/978-1-0716-1311-5_18
doi:
Substances chimiques
CCR5 protein, human
0
RNA, Messenger
0
Receptors, CCR5
0
Transcription Activator-Like Effector Nucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
217-226Références
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