Selection of Chemical Modifications in the siRNA Seed Region That Repress Off-Target Effect.
2′-OMe
Off-target effect
RNAi
Seed region
siRNA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
24
6
2021
Statut:
ppublish
Résumé
RNA interference mediated by small interfering RNA (siRNA) has been widely used as a procedure to knock down the expression of an intended target gene with perfect sequence complementarity. However, siRNA often exhibits off-target effects on genes with partial sequence complementarities. Such off-target effect is an undesirable adverse effect for knocking down a target gene specifically. Here we describe the powerful strategy to avoid off-target effects without affecting the RNAi activity by the introduction of DNA or 2'-O-methyl modifications in the siRNA seed region. These two types of chemical modifications repress off-target effects through different molecular mechanisms.
Identifiants
pubmed: 33928567
doi: 10.1007/978-1-0716-1298-9_2
doi:
Substances chimiques
RNA, Small Interfering
0
VIM protein, human
0
Vimentin
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17-30Références
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