siRNA Vehicles for High Endosomal Escapability.

Cell Line, Tumor Endosomes / metabolism Fibrosarcoma / genetics Gene Expression Regulation, Neoplastic Gene Transfer Techniques Humans Hydrogen-Ion Concentration Liposomes / chemistry RNA Interference RNA Stability RNA, Small Interfering / chemistry Research Design Workflow
Confocal image Endosome Lipid nanoparticle Small interfering RNA pH-responsive vehicle

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2021
Historique:
entrez: 30 4 2021
pubmed: 1 5 2021
medline: 24 6 2021
Statut: ppublish

Résumé

Small interfering RNA (siRNA) is a novel therapeutic modality for the treatment of intractable diseases; however, the development of a useful siRNA delivery vector is imperative for clinical use. Since siRNA works in the cytoplasm, the ability of the carrier to escape destruction in the endosomes is a highly required characteristic for the induction of a high knockdown effect. Here, we describe the step-by-step procedure for the evaluation of high endosomal escapability. The vector that has pH-responsive characteristics at around pH = 6.2-6.5 is important for the high endosomal escape.

Identifiants

DOI: 10.1007/978-1-0716-1298-9_11 PMID: 33928576
pubmed: 33928576
doi: 10.1007/978-1-0716-1298-9_11
doi:

Substances chimiques

Liposomes 0
RNA, Small Interfering 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

171-179

Références

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doi: 10.1038/nrd2310 pubmed: 17541417 pmcid: 7098199
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doi: 10.1016/j.bbrc.2007.10.116 pubmed: 17971296
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doi: 10.1038/nbt.3802 pubmed: 28244992
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doi: 10.1021/acs.biomac.9b00611 pubmed: 31518109
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doi: 10.1073/pnas.1306529110 pubmed: 23882076 pmcid: 3740846

Auteurs

Hiroyuki Koide (H)

Department of Medical Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan.

Sei Yonezawa (S)

Department of Medical Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan.

Tomohiro Asai (T)

Department of Medical Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan. asai@u-shizuoka-ken.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Vésicules de transport › Endosomes › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Tumeurs du tissu conjonctif et des tissus mous › Sarcomes › Fibrosarcome › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes tumoraux › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de transfert de gènes › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité de l'ARN › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › Petit ARN interférent › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Disciplines des sciences naturelles › Science › Recherche › Plan de recherche › siRNA Vehicles for High Endosomal Escapability.
questionsmedicales.fr › Sciences de l'information › Analyse des systèmes › Flux de travaux › siRNA Vehicles for High Endosomal Escapability.