Cross-talk between motor neurons and myotubes via endogenously secreted neural and muscular growth factors.
cross-talk
motor neurons
muscle
neural growth factors
neuromuscular junction (NMJ)
Journal
Physiological reports
ISSN: 2051-817X
Titre abrégé: Physiol Rep
Pays: United States
ID NLM: 101607800
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
revised:
11
02
2021
received:
09
12
2020
accepted:
12
02
2021
entrez:
1
5
2021
pubmed:
2
5
2021
medline:
5
1
2022
Statut:
ppublish
Résumé
Neuromuscular junction (NMJ) research is vital to advance the understanding of neuromuscular patho-physiology and development of novel therapies for diseases associated with NM dysfunction. In vivo, the micro-environment surrounding the NMJ has a significant impact on NMJ formation and maintenance via neurotrophic and differentiation factors that are secreted as a result of cross-talk between muscle fibers and motor neurons. Recently we showed the formation of functional NMJs in vitro in a co-culture of immortalized human myoblasts and motor neurons from rat-embryo spinal-cord explants, using a culture medium free from serum and neurotrophic or growth factors. The aim of this study was to assess how functional NMJs were established in this co-culture devoid of exogenous neural growth factors. To investigate this, an ELISA-based microarray was used to compare the composition of soluble endogenously secreted growth factors in this co-culture with an a-neural muscle culture. The levels of seven neurotrophic factors brain-derived neurotrophic factor (BDNF), glial-cell-line-derived neurotrophic factor (GDNF), insulin-like growth factor-binding protein-3 (IGFBP-3), insulin-like growth factor-1 (IGF-1), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), and vascular endothelial growth factor (VEGF) were higher (p < 0.05) in the supernatant of NMJ culture compared to those in the supernatant of the a-neural muscle culture. This indicates that the cross-talk between muscle and motor neurons promotes the secretion of soluble growth factors contributing to the local microenvironment thereby providing a favourable regenerative niche for NMJs formation and maturation.
Identifiants
pubmed: 33931983
doi: 10.14814/phy2.14791
pmc: PMC8087923
doi:
Substances chimiques
Nerve Growth Factors
0
Vascular Endothelial Growth Factor A
0
Insulin-Like Growth Factor I
67763-96-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14791Informations de copyright
© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.
Références
J Neurosci. 2003 Jun 15;23(12):5050-60
pubmed: 12832528
Eur J Neurosci. 2005 Apr;21(8):2100-10
pubmed: 15869506
Front Cell Neurosci. 2015 Dec 08;9:473
pubmed: 26696831
Eur J Neurosci. 1995 May 1;7(5):1107-10
pubmed: 7613616
Front Cell Neurosci. 2014 Feb 28;8:61
pubmed: 24616665
Endocrinology. 1993 Sep;133(3):1462-5
pubmed: 7689959
Cells Tissues Organs. 2016;202(3-4):143-158
pubmed: 27825148
Muscle Nerve. 2006 Apr;33(4):462-76
pubmed: 16228973
J Biomed Biotechnol. 2011;2011:201696
pubmed: 21960735
J Biol Chem. 2002 Mar 22;277(12):10614-25
pubmed: 11790765
J Neurosci Res. 2000 Feb 15;59(4):553-60
pubmed: 10679795
Neuroscience. 2007 May 11;146(2):481-8
pubmed: 17383103
Exp Neurol. 1996 Oct;141(2):297-303
pubmed: 8812163
Muscle Nerve. 2004 Mar;29(3):381-6
pubmed: 14981737
Biomaterials. 2010 Nov;31(32):8218-27
pubmed: 20708792
Front Cell Neurosci. 2014 Dec 10;8:405
pubmed: 25540609
Neurosci Lett. 1998 Jul 3;250(2):87-90
pubmed: 9697925
Organogenesis. 2010 Apr-Jun;6(2):107-14
pubmed: 20885857
Front Physiol. 2019 Jan 30;10:42
pubmed: 30761018
Physiol Rev. 2015 Jul;95(3):809-52
pubmed: 26109340
Stem Cells Cloning. 2018 Nov 09;11:85-93
pubmed: 30519053
Physiol Rev. 2009 Apr;89(2):607-48
pubmed: 19342615
Endocr Rev. 1999 Dec;20(6):761-87
pubmed: 10605625
Biomaterials. 2011 Dec;32(36):9602-11
pubmed: 21944471
Diabetologia. 1998 Mar;41(3):300-6
pubmed: 9541170
Development. 2016 Jul 1;143(13):2464-77
pubmed: 27226316
Neuron. 1999 Nov;24(3):567-83
pubmed: 10595510
J Neurosci. 2006 May 24;26(21):5739-49
pubmed: 16723531
J Neurosci. 2007 Sep 26;27(39):10350-64
pubmed: 17898207
Biomaterials. 2010 Jun;31(18):4880-8
pubmed: 20346499
J Neurosci. 2008 Jan 23;28(4):963-75
pubmed: 18216204
Cell Tissue Res. 2006 Nov;326(2):263-71
pubmed: 16819627
J Exp Biol. 2003 Jul;206(Pt 13):2191-200
pubmed: 12771168
Stem Cells Cloning. 2019 Feb 20;12:1-9
pubmed: 30863121
World J Gastroenterol. 2017 May 21;23(19):3488-3495
pubmed: 28596684
Mol Neurobiol. 2000 Jun;21(3):109-35
pubmed: 11379795
J Neurosci. 2001 Aug 15;21(16):6136-46
pubmed: 11487637
J Neurosci Res. 1995 Sep 1;42(1):21-33
pubmed: 8531223
Biomaterials. 2017 Apr;122:179-187
pubmed: 28129596
Biochem Biophys Res Commun. 2007 Nov 30;363(4):989-93
pubmed: 17923114
Science. 1995 Jun 9;268(5216):1495-9
pubmed: 7770776
J Neurobiol. 1993 Dec;24(12):1555-77
pubmed: 8301265
Mol Cell Neurosci. 2001 Jul;18(1):56-67
pubmed: 11461153
Nat Rev Cancer. 2013 Oct;13(10):714-26
pubmed: 24060863
Science. 1993 May 21;260(5111):1130-2
pubmed: 8493557
Neuron. 2002 Nov 14;36(4):675-88
pubmed: 12441056
Endocr Rev. 2004 Aug;25(4):581-611
pubmed: 15294883
Nature. 1995 Jan 26;373(6512):344-6
pubmed: 7830769
J Peripher Nerv Syst. 2005 Sep;10(3):293-300
pubmed: 16221288
Neurosci Lett. 2015 Mar 30;591:8-12
pubmed: 25681622
Mol Biol Cell. 2010 Jul 1;21(13):2182-90
pubmed: 20427568
Biomater Sci. 2014 Jan 1;2(1):131-138
pubmed: 24516722
J Clin Med. 2020 Oct 10;9(10):
pubmed: 33050427
Neurosci Lett. 1999 Sep 10;272(2):107-10
pubmed: 10507553
Biol Cell. 1986;57(1):17-22
pubmed: 2945607
Nat Rev Drug Discov. 2018 Mar;17(3):183-196
pubmed: 29217837
Mol Ther. 2014 Jul;22(7):1243-1253
pubmed: 24769910
J Neurosci Res. 2010 May 15;88(7):1406-19
pubmed: 20029969
Cell. 1994 May 20;77(4):503-12
pubmed: 7514502
J Neurochem. 1995 Mar;64(3):1231-7
pubmed: 7861156
J Cell Biol. 2005 Jan 17;168(2):193-9
pubmed: 15657392
J Physiol. 2006 Jan 15;570(Pt 2):283-94
pubmed: 16293644
Annu Rev Neurosci. 2001;24:677-736
pubmed: 11520916
Science. 1998 Mar 13;279(5357):1725-9
pubmed: 9497292
Br J Pharmacol. 2008 Jun;154(3):557-68
pubmed: 18500379
Neuropeptides. 1996 Aug;30(4):309-12
pubmed: 8914855
Eur J Neurosci. 2010 Jan;31(1):29-41
pubmed: 20092553