Improving the Thermostability and Activity of Transaminase From

amine transaminase enzyme thermal stability system molecular dynamics simulations site-directed mutagenesis thermostability

Journal

Frontiers in chemistry
ISSN: 2296-2646
Titre abrégé: Front Chem
Pays: Switzerland
ID NLM: 101627988

Informations de publication

Date de publication:
2021
Historique:
received: 05 02 2021
accepted: 23 03 2021
entrez: 3 5 2021
pubmed: 4 5 2021
medline: 4 5 2021
Statut: epublish

Résumé

Transaminases that promote the amination of ketones into amines are an emerging class of biocatalysts for preparing a series of drugs and their intermediates. One of the main limitations of (

Identifiants

pubmed: 33937200
doi: 10.3389/fchem.2021.664156
pmc: PMC8081293
doi:

Types de publication

Journal Article

Langues

eng

Pagination

664156

Informations de copyright

Copyright © 2021 Cao, Fan, Lv, Wang, Li, Hu, Zhao, Chen, Huang and Mei.

Déclaration de conflit d'intérêts

H-BC was employed by the company Enzymaster (Ningbo) Bio-Engineering. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Références

J Mol Biol. 2004 Feb 27;336(4):929-42
pubmed: 15095870
Br J Anaesth. 2002 Apr;88(4):563-76
pubmed: 12066734
Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):1274-1279
pubmed: 29358381
Biochemistry. 2017 Dec 19;56(50):6521-6532
pubmed: 29087185
European J Org Chem. 2015 Nov;2015(32):6965-6982
pubmed: 26726292
Methods Mol Biol. 2009;490:261-83
pubmed: 19157087
J Mol Biol. 2001 Sep 28;312(4):885-96
pubmed: 11575940
Biochemistry. 1999 Jun 22;38(25):8138-49
pubmed: 10387059
Biochemistry. 2006 Mar 7;45(9):2761-6
pubmed: 16503630
Bioorg Med Chem Lett. 2011 Jan 1;21(1):383-6
pubmed: 21087862
Biochem Biophys Res Commun. 2017 Jan 29;483(1):397-402
pubmed: 28017723
J Biotechnol. 2019 Mar 10;293:8-16
pubmed: 30703468
Org Biomol Chem. 2012 Mar 28;10(12):2482-5
pubmed: 22344532
Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2601-6
pubmed: 19196981
CRC Crit Rev Biochem. 1985;18(2):91-197
pubmed: 3899508
Appl Microbiol Biotechnol. 2014 Jan;98(2):651-60
pubmed: 23576035
Org Process Res Dev. 2013 May 17;17(5):751-759
pubmed: 23794796
PLoS One. 2014 Jan 30;9(1):e87350
pubmed: 24498081
J Mol Biol. 2003 Apr 11;327(5):1135-48
pubmed: 12662936
Methods Enzymol. 1986;130:437-53
pubmed: 3773743
J Mol Biol. 1984 Sep 5;178(1):63-89
pubmed: 6548264
J Ind Microbiol Biotechnol. 2014 Aug;41(8):1217-25
pubmed: 24879480
Protein Eng Des Sel. 2007 Nov;20(11):551-9
pubmed: 17977846
Enzyme Microb Technol. 2020 Sep;139:109603
pubmed: 32732025
Nat Chem. 2017 Oct;9(10):961-969
pubmed: 28937665
Nature. 2012 May 09;485(7397):185-94
pubmed: 22575958
Biotechnol J. 2009 Oct;4(10):1420-31
pubmed: 19757497
Biochemistry. 1979 May 15;18(10):1919-28
pubmed: 435457
Nat Protoc. 2007;2(9):2212-21
pubmed: 17853878
Biotechnol Bioeng. 2011 Jul;108(7):1479-93
pubmed: 21455931
Org Lett. 2014 Dec 5;16(23):6092-5
pubmed: 25394227
J Med Chem. 2004 May 20;47(11):2887-96
pubmed: 15139767
J Mol Biol. 2011 May 6;408(3):514-28
pubmed: 21377472
World J Microbiol Biotechnol. 2017 Dec 18;34(1):13
pubmed: 29255954
Appl Microbiol Biotechnol. 2012 Jun;94(5):1163-71
pubmed: 22555915
Biotechnol Appl Biochem. 2018 Mar;65(2):255-262
pubmed: 28639260
Protein Sci. 2014 Jan;23(1):110-6
pubmed: 24353171
J Biotechnol. 2018 Jul 20;278:1-9
pubmed: 29660473
Methods Enzymol. 1986;130:413-36
pubmed: 3773742
Trends Biotechnol. 2001 Apr;19(4):132-5
pubmed: 11250029
Curr Opin Chem Biol. 2018 Apr;43:106-112
pubmed: 29278779
Struct Dyn. 2019 Mar 25;6(2):024702
pubmed: 31111079
Anal Chem. 2009 Oct 1;81(19):8244-8
pubmed: 19739593
Appl Biochem Biotechnol. 2020 Oct;192(2):530-543
pubmed: 32405732
Angew Chem Int Ed Engl. 2004 Feb 6;43(7):788-824
pubmed: 14767950
Molecules. 2019 Mar 27;24(7):
pubmed: 30934681

Auteurs

Jia-Ren Cao (JR)

School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, China.

Fang-Fang Fan (FF)

School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, China.

Chang-Jiang Lv (CJ)

School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, China.

Hong-Peng Wang (HP)

School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, China.

Ye Li (Y)

School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, China.

Sheng Hu (S)

School of Biotechnology and Chemical Engineering, NingboTech University, Ningbo, China.

Wei-Rui Zhao (WR)

School of Biotechnology and Chemical Engineering, NingboTech University, Ningbo, China.

Hai-Bin Chen (HB)

Enzymaster (Ningbo) Bio-Engineering Co., Ltd., Ningbo, China.

Jun Huang (J)

School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, China.

Le-He Mei (LH)

School of Biotechnology and Chemical Engineering, NingboTech University, Ningbo, China.
Jinhua Advanced Research Institute, Jinhua, China.
Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.

Classifications MeSH