Therapeutic antibodies, targeting the SARS-CoV-2 spike N-terminal domain, protect lethally infected K18-hACE2 mice.
Immunology
Molecular biology
Virology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
21 May 2021
21 May 2021
Historique:
received:
04
02
2021
revised:
24
03
2021
accepted:
23
04
2021
pubmed:
4
5
2021
medline:
4
5
2021
entrez:
3
5
2021
Statut:
ppublish
Résumé
Neutralizing antibodies represent a valuable therapeutic approach to countermeasure the current COVID-19 pandemic. Emergence of SARS-CoV-2 variants emphasizes the notion that antibody treatments need to rely on highly neutralizing monoclonal antibodies (mAbs), targeting several distinct epitopes for circumventing therapy escape mutants. Previously, we reported efficient human therapeutic mAbs recognizing epitopes on the spike receptor-binding domain (RBD) of SARS-CoV-2. Here we report the isolation, characterization, and recombinant production of 12 neutralizing human mAbs, targeting three distinct epitopes on the spike N-terminal domain of the virus. Neutralization mechanism of these antibodies involves receptors other than the canonical hACE2 on target cells, relying both on amino acid and
Identifiants
pubmed: 33937725
doi: 10.1016/j.isci.2021.102479
pii: S2589-0042(21)00447-8
pmc: PMC8074524
doi:
Types de publication
Journal Article
Langues
eng
Pagination
102479Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
Patent application for the described antibodies was filed by the Israel Institute for Biological Research. None of the authors declared any additional competing interests.
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