Effects of lifestyle interventions on epigenetic signatures of liver fat: Central randomized controlled trial.
DNA-methylation
Diet
genetic variation
nonalcoholic fatty liver disease
physical activity
Journal
Liver international : official journal of the International Association for the Study of the Liver
ISSN: 1478-3231
Titre abrégé: Liver Int
Pays: United States
ID NLM: 101160857
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
13
04
2021
received:
21
03
2021
accepted:
24
04
2021
pubmed:
4
5
2021
medline:
20
8
2021
entrez:
3
5
2021
Statut:
ppublish
Résumé
In the CENTRAL trial context, we found diverse liver fat dynamics in response to different dietary interventions. Epigenetic mechanisms may contribute to the intraindividual variation. Moreover, genetic factors are involved in developing nonalcoholic fatty-liver disease (NAFLD), a disease reflected by an increase in intrahepatic fat (IHF). In this exploratory analysis, we primarily aimed to examine the effect of lifestyle interventions on DNA-methylation of NAFLD related genes associated with IHF. For 120 participants from the CENTRAL trial, an 18-month regimen of either low-fat (LF) or Mediterranean-low carbohydrate (MED/LC) diets, with or without physical activity (PA+/PA-), was instructed. Magnetic resonance imaging was used to measure IHF%, which was analysed for association with CpG specific DNA-methylation levels of 41 selected candidate genes. Single-nucleotide polymorphisms known to be associated with NAFLD within the studied genes were genotyped by TaqMan assays. At baseline, participants (92% men; body mass index = 30.2 kg/m This study suggests epigenetic markers for IHF and potential epigenetic remodeling after long-term lifestyle interventions.
Sections du résumé
BACKGROUND AND AIMS
In the CENTRAL trial context, we found diverse liver fat dynamics in response to different dietary interventions. Epigenetic mechanisms may contribute to the intraindividual variation. Moreover, genetic factors are involved in developing nonalcoholic fatty-liver disease (NAFLD), a disease reflected by an increase in intrahepatic fat (IHF). In this exploratory analysis, we primarily aimed to examine the effect of lifestyle interventions on DNA-methylation of NAFLD related genes associated with IHF.
METHODS
For 120 participants from the CENTRAL trial, an 18-month regimen of either low-fat (LF) or Mediterranean-low carbohydrate (MED/LC) diets, with or without physical activity (PA+/PA-), was instructed. Magnetic resonance imaging was used to measure IHF%, which was analysed for association with CpG specific DNA-methylation levels of 41 selected candidate genes. Single-nucleotide polymorphisms known to be associated with NAFLD within the studied genes were genotyped by TaqMan assays.
RESULTS
At baseline, participants (92% men; body mass index = 30.2 kg/m
CONCLUSIONS
This study suggests epigenetic markers for IHF and potential epigenetic remodeling after long-term lifestyle interventions.
Substances chimiques
CRACR2A protein, human
0
Calcium-Binding Proteins
0
Banques de données
ClinicalTrials.gov
['NCT01530724']
Types de publication
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2101-2111Informations de copyright
© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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