Trihydroxycholanoyl-taurine in brains of rodents with hepatic encephalopathy.
brain
hepatic encephalopathy
mass spectrometry
taurocholic acid
trihydroxycholanoyl-taurine
Journal
Journal of mass spectrometry : JMS
ISSN: 1096-9888
Titre abrégé: J Mass Spectrom
Pays: England
ID NLM: 9504818
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
revised:
22
03
2021
received:
14
01
2021
accepted:
01
04
2021
entrez:
4
5
2021
pubmed:
5
5
2021
medline:
16
11
2021
Statut:
ppublish
Résumé
Hepatic encephalopathy (HE), a neurological disease resulting from liver failure, is difficult to manage and its causes are unclear. Bile acids have been postulated to be involved in the provenance and progression of various diseases including HE. Hence, the characterization of bile acid profiles in the brains of subjects with and without liver failure can provide important clues for the potential treatment of HE. Nanoflow ultra-performance liquid chromatography electrospray ionization ion mobility mass spectrometry (UPLC-ESI-IM-MS) is a highly sensitive method for detection of specific molecules, such as bile acids in brain samples, at biologically relevant concentrations. We used UPLC-ESI-IM-MS to characterize bile acid profiles in brain samples from seven "healthy" control rodents and 22 "diseased" rodents with liver failure (i.e., induced HE). An isomer of trihydroxycholanoyl-taurine was detected in brain tissue samples from both rats and mice with induced HE; however, this isomer was not detected in the brains of healthy rats and mice. Our findings were confirmed by comparing IM arrival times (AT), exact mass measurements (m/z), and mass spectral fragmentation patterns of the experimentally observed suspected species to standards of trihydroxycholanoyl-taurine isomers. Moreover, In Silico Fractionation was employed to provide an additional analytical dimension to verify bile acid identifications.
Substances chimiques
Bile Acids and Salts
0
Taurine
1EQV5MLY3D
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4729Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK112803
Pays : United States
Organisme : United States Department of Veterans Affairs Biomedical Laboratory Research and Development Service
ID : BX002638
Organisme : United States Department of Veterans Affairs Biomedical Laboratory Research and Development Service
ID : DK082435
Informations de copyright
© 2021 John Wiley & Sons Ltd. This article has been contributed to by US Government employees and their work is in the public domain in the USA.
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