Mini-patient-derived xenograft assay based on microfluidic technology promises to be an effective tool for screening individualized chemotherapy regimens for advanced non-small cell lung cancer.
Aged
Antineoplastic Combined Chemotherapy Protocols
/ pharmacology
Carcinoma, Non-Small-Cell Lung
/ drug therapy
Drug Resistance, Neoplasm
/ drug effects
Female
Humans
Lung Neoplasms
/ drug therapy
Male
Microfluidic Analytical Techniques
/ methods
Middle Aged
Xenograft Model Antitumor Assays
/ methods
individualized chemotherapy regimens
microfluidic technology
mini-patient-derived xenograft assay
non-small cell lung cancer
Journal
Cell biology international
ISSN: 1095-8355
Titre abrégé: Cell Biol Int
Pays: England
ID NLM: 9307129
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
08
04
2021
received:
11
01
2021
accepted:
01
05
2021
pubmed:
5
5
2021
medline:
11
1
2022
entrez:
4
5
2021
Statut:
ppublish
Résumé
Patient-derived xenograft (PDX) assay has been widely used in preclinical research in patients with multidrug-resistant lung cancer. One hundred patients with non-small cell lung cancer (NSCLC) were divided into MiniPDX group and conventional group, with 50 cases in each group. The MiniPDX assay was established by enriching high-purity tumor cells using microfluidic technology to detect the drug sensitivity of NSCLC cells. All patients underwent conventional computed tomography (CT) scans of lung and mediastinum at baseline and during follow-up. Kaplan-Meier method was used to compare the overall survival and progression-free survival of two groups. The sensitivity of the same drug in different tumor xenograft varied greatly. The overall survival, progression-free survival, and clinical benefit rate of patients in the MiniPDX-guided chemotherapy group were significantly longer than those in the conventional chemotherapy group. MiniPDX assay may be an effective tool for screening chemotherapy regimens in NSCLC patients.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1887-1896Subventions
Organisme : Shanghai Chest Hospital Project
ID : 2019YNJCM06
Organisme : Shanghai Chest Hospital Project of Collaborative Innovation Grant
ID : YJXT20190210
Organisme : the Foundation of Shanghai Shen Kang Hospital Development Center
ID : 16CR3071B
Organisme : Science and Technology Commission of Shanghai Municipality Project
ID : 19140902600
Organisme : China Center for Evidence Based Traditional Chinese Medicine
ID : ZXZL-6
Informations de copyright
© 2021 International Federation for Cell Biology.
Références
Ben-David, U. , Ha, G. , Tseng, Y. Y. , Greenwald, N. F. , Oh, C. , Shih, J. , Mcfarland, J. M. , Wong, B. , Boehm, J. S. , Beroukhim, R. , & Golub, T. R. (2017). Patient-derived xenografts undergo mouse-specific tumor evolution. Nature Genetics, 49(11), 1567-1575. https://doi.org/10.1038/ng.3967
Bissig-Choisat, B. , Kettlun-Leyton, C. , Legras, X. D. , Zorman, B. , Barzi, M. , Chen, L. L. , Amin, M. D. , Huang, Y. H. , Pautler, R. G. , Hampton, O. A. , Prakash, M. M. , Yang, D. , Borowiak, M. , Muzny, D. , Doddapaneni, H. V. , Hu, J. , Shi, Y. , Gaber, M. W. , Hicks, M. J. , … Bissig, K. D. (2016). Novel patient-derived xenograft and cell line models for therapeutic testing of pediatric liver cancer. Journal of Hepatology, 65(2), 325-333.
Byrne, A. T. , Alférez, D. G. , Amant, F. , Annibali, D. , Arribas, J. , Biankin, A. V. , Bruna, A. , Budinská, E. , Caldas, C. , Chang, D. K. , Clarke, R. B. , Clevers, H. , Coukos, G. , Dangles-Marie, V. , Eckhardt, S. G. , Gonzalez-Suarez, E. , Hermans, E. , Hidalgo, M. , Jarzabek, M. A. , … Trusolino, L. (2017). Interrogating open issues in cancer precision medicine with patient-derived xenografts. Nature Reviews Cancer, 17(4), 254-268. https://doi.org/10.1038/nrc.2016.140
Chapuy, B. , Cheng, H. , Watahiki, A. , Ducar, M. D. , Tan, Y. , Chen, L. , Roemer, M. G. , Ouyang, J. , Christie, A. L. , Zhang, L. , Gusenleitner, D. , Abo, R. P. , Farinha, P. , von Bonin, F. , Thorner, A. R. , Sun, H. H. , Gascoyne, R. D. , Pinkus, G. S. , van Hummelen, P. , … Shipp, M. A. (2016). Diffuse large B-cell lymphoma patient-derived xenograft models capture the molecular and biological heterogeneity of the disease. Blood, 127(18), 2203-2213. https://doi.org/10.1182/blood-2015-09-672352
Durm, G. , & Hanna, N. (2017). Second-line chemotherapy and beyond for non-small cell lung cancer. Hematology/Oncology Clinics of North America, 31(1), 71-81. https://doi.org/10.1016/j.hoc.2016.08.002
Ettinger, D. S. , Akerley, W. , Borghaei, H. , Chang, A. C. , Cheney, R. T. , Chirieac, L. R. , D'Amico, T. A. , Demmy, T. L. , Govindan, R. , Grannis, F. W., Jr. , Grant, S. C. , Horn, L. , Jahan, T. M. , Komaki, R. , Kong, F.-M. S. , Kris, M. G. , Krug, L. M. , Lackner, R. P. , Lennes, I. T. , … Hughes, M. (2013). Non-small cell lung cancer, version 2.2013. Journal of the National Comprehensive Cancer Network, 11(6), 645-653. https://doi.org/10.6004/jnccn.2013.0084
Gandara, D. R. , Lara, P. N. , & Mack, P. C. (2015). Patient-derived xenografts for investigation of acquired resistance in oncogene-driven cancers: Building a better mousetrap. Journal of Clinical Oncology Official Journal of the American Society of Clinical Oncology, 33(26), 2839-2840.
Gridelli, C. , Chen, T. , Ko, A. , O'Brien, M. E. , Teng, J. O. , Socinski, M. A. , & Postmus, P. E. (2018). nab-Paclitaxel/carboplatin in elderly patients with advanced squamous non-small cell lung cancer: A retrospective analysis of a Phase III trial. Drug Design Development & Therapy, 12, 1445-1451.
He, M. , & Zeng, Y. (2016). Microfluidic exosome analysis toward liquid biopsy for cancer. Journal of Laboratory Automation, 21(4), 599-608.
Hidalgo, M. , Amant, F. , Biankin, A. V. , Budinská, E. , Byrne, A. T. , Caldas, C. , Clarke, R. B. , de Jong, S. , Jonkers, J. , Maelandsmo, G. M. , Roman-Roman, S. , Seoane, J. , Trusolino, L. , & Villanueva, A. (2014). Patient-derived xenograft models: An emerging platform for translational cancer research. Cancer Discovery, 4(9), 998-1013. https://doi.org/10.1158/2159-8290.cd-14-0001
Hu, X. , Chen, L. , Du, Y. , Fan, B. , Bu, Z. , Wang, X. , Ye, Y. , Zhang, Z. , Xiao, G. , Li, F. , He, Q. , Li, G. , Shen, X. , Xiong, B. , Zhu, L. , Liu, J. , Liu, L. , Wu, T. , Zhou, J. , … Ji, J. (2017). Postoperative chemotherapy with S-1 plus oxaliplatin versus S-1 alone in locally advanced gastric cancer (RESCUE-GC study): A protocol for a phase III randomized controlled trial. Chinese Journal of Cancer Research, 29(2), 144-148. https://doi.org/10.21147/j.issn.1000-9604.2017.02.07
Isler, J. , Katz, A. , Sorogin, A. , Scoppetuolo, M. , Nechushtan, H. , Pass, H. , Davies, A. , & Paz, K. (2014). The use of patient-derived xenograft (PDX) models to predict patient response in Non-Small Cell Lung Cancer (NSCLC): Metastatic non-small cell lung cancer. International Journal of Radiation Oncology, Biology, Physics, 90(5), S42-S43.
Kim, H. S. , Kim, J. H. , Kim, B. , Choi, H. C. , Kwon, J. H. , & Choi, D. R. (2013). Phase II study of weekly carboplatin and irinotecan as first-line;chemotherapy for patients with advanced non-small cell lung cancer. Cancer Chemotherapy & Pharmacology, 71(6), 1591-1597.
Lorz, A. , Lorenzi, T. , Clairambault, J. , Escargueil, A. , & Perthame, B. (2015). Modeling the effects of space structure and combination therapies on phenotypic heterogeneity and drug resistance in solid tumors. Bulletin of Mathematical Biology, 77(1), 1-22. https://doi.org/10.1007/s11538-014-0046-4
Nakanishi, T. , Menju, T. , Nishikawa, S. , Takahashi, K. , Miyata, R. , Shikuma, K. , Sowa, T. , Imamura, N. , Hamaji, M. , Motoyama, H. , Hijiya, K. , Aoyama, A. , Sato, T. , Chen-Yoshikawa, T. F. , Sonobe, M. , & Date, H. (2018). The synergistic role of ATP-dependent drug efflux pump and focal adhesion signaling pathways in vinorelbine resistance in lung cancer. Cancer Medicine, 7(2), 408-419.
Nicolle, D. , Fabre, M. , Simon-Coma, M. , Gorse, A. , Kappler, R. , Nonell, L. , Mallo, M. , Haidar, H. , Déas, O. , Mussini, C. , Guettier, C. , Redon, M. J. , Brugières, L. , Ghigna, M. R. , Fadel, E. , Galmiche-Rolland, L. , Chardot, C. , Judde, J. G. , Armengol, C. , … Cairo, S. (2016). Patient-derived mouse xenografts from pediatric liver cancer predict tumor recurrence and advise clinical management. Hepatology, 64(4), 1121-1135. https://doi.org/10.1002/hep.28621
Pei, H. , Li, L. , Han, Z. , Wang, Y. , & Tang, B. (2020). Recent advances in microfluidic technologies for circulating tumor cells: Enrichment, single-cell analysis, and liquid biopsy for clinical applications. Lab on a Chip, 20(21), 3854-3875. https://doi.org/10.1039/d0lc00577k
Qin, S. , Yu, H. , Wu, X. , Luo, Z. , Wang, H. , Sun, S. , Huang, M. , Jin, J. , Tao, Z. , Qiao, J. , Feng, Y. , Wang, J. , & Chang, J. (2019). Weekly albumin-bound paclitaxel/cisplatin versus gemcitabine/cisplatin as first-line therapy for patients with advanced non-small-cell lung cancer: A phase II open-label clinical study. Chinese Journal of Cancer Research, 31(2), 339-348. https://doi.org/10.21147/j.issn.1000-9604.2019.02.08
Rana, A. , Zhang, Y. , & Esfandiari, L. (2018). Advancements in microfluidic technologies for isolation and early detection of circulating cancer-related biomarkers. The Analyst, 143(13), 2971-2991. https://doi.org/10.1039/c7an01965c
Shen, Q. , Peng, C. , Zhan, Y. , Fan, L. , Wang, M. , Zhou, Q. , Liu, J. , Lv, X. , Tang, Q. , Li, J. , Huang, X. , & Xia, J. (2016). Aptamer-polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing. International Journal of Nanomedicine, 11, 2133-2146. https://doi.org/10.2147/ijn.s103569
Siegel, R. L. , Miller, K. D. , & Jemal, A. (2018). Cancer statistics, 2018. CA: A Cancer Journal for Clinicians, 68(1), 7-30. https://doi.org/10.3322/caac.21442
Xiang Yong, X. U. , Wang, Q. , Ping, X. U. , & Wang, X. (2017). Expression of ERCC1 and BRCA1 in tissues of advanced non-small-cell lung cancer and its relationship with the sensitivity of platinum based chemotherapy. Chinese Journal of Cancer Prevention & Treatment, 24(3), 178-182.
Xing Sheng, H. , Lin, W. , Lin, L. , Xiaohong, H. , Guifang, D. , Zhiyun, M. , & Yuankai, S. (2016). Aphase I trial of anoral sub type-selective histone deacetylaseinhibitor, chidamide, in combination with paclitaxel and carboplatin inpatients with advanced non-small celllungcancer. Chinese Journal of Cancer Research, 28(4), 444-451.
Zhan, M. , Yang, R. M. , Wang, H. , He, M. , Chen, W. , Xu, S. W. , Yang, L. H. , Liu, Q. , Long, M. M. , & Wang, J. (2018). Guided chemotherapy based on patient-derived mini-xenograft models improves survival of gallbladder carcinoma patients. Cancer Communications, 38(1), 48. https://doi.org/10.1186/s40880-018-0318-8
Zhou, H. , Song, Y. , Jiang, J. , Niu, H. , Zhao, H. , Liang, J. , Su, H. , Wang, Z. , Zhou, Z. , & Huang, J. (2016). A pilot phase II study of neoadjuvant triplet chemotherapy regimen in patients with locally advanced resectable colon cancer. Chinese Journal of Cancer Research, 28(6), 598-605.