An Optimized Preparation Method for Long ssDNA Donors to Facilitate Quick Knock-In Mouse Generation.

Animals CRISPR-Cas Systems DNA, Single-Stranded / chemistry Electroporation / methods Gene Editing / methods Gene Knock-In Techniques / methods Mice Mice, Transgenic Microinjections / methods Polymerase Chain Reaction / methods Zygote / metabolism
CRISPR/Cas9 knock-in long ssDNA phospho-PCR

Journal

Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052

Informations de publication

Date de publication:
30 04 2021
Historique:
received: 28 03 2021
revised: 22 04 2021
accepted: 28 04 2021
entrez: 5 5 2021
pubmed: 6 5 2021
medline: 25 2 2023
Statut: epublish

Résumé

Fluorescent reporter mouse lines and Cre/Flp recombinase driver lines play essential roles in investigating various molecular functions

Identifiants

DOI: 10.3390/cells10051076 PMID: 33946570 PMC: PMC8147208
pubmed: 33946570
pii: cells10051076
doi: 10.3390/cells10051076
pmc: PMC8147208
pii:
doi:

Substances chimiques

DNA, Single-Stranded 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Références

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Auteurs

Yukiko U Inoue (YU)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.
ORCID: 0000-0002-4105-9127

Yuki Morimoto (Y)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Mayumi Yamada (M)

Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.

Ryosuke Kaneko (R)

KOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.

Kazumi Shimaoka (K)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Shinji Oki (S)

Department of Immunology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Mayuko Hotta (M)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Junko Asami (J)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Eriko Koike (E)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Kei Hori (K)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Mikio Hoshino (M)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.

Itaru Imayoshi (I)

Research Center for Dynamic Living Systems, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.
Department of Deconstruction of Stem Cells, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.

Takayoshi Inoue (T)

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.
ORCID: 0000-0001-7155-2150

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Classifications MeSH

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questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électroporation › An Optimized Preparation Method for Long ssDNA...
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