Phenotypic Characteristics of the Tumour Microenvironment in Primary and Secondary Hepatocellular Carcinoma.
hepatocellular carcinoma
intra-tumoural heterogeneity
tumour microenvironment
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
29 Apr 2021
29 Apr 2021
Historique:
received:
27
03
2021
revised:
24
04
2021
accepted:
25
04
2021
entrez:
5
5
2021
pubmed:
6
5
2021
medline:
6
5
2021
Statut:
epublish
Résumé
(1) Background: The intra-tumoural heterogeneity (ITH) of hepatocellular carcinoma (HCC) and its microenvironment (TME) across primary and secondary disease is poorly characterised. (2) Methods: Intra-tumoural (IT) and peri-tumoural (PT) staining of matched primary and secondary samples was conducted to evaluate the distribution of CD4+/FOXP3+ and CD8+/PD1+ T-cells. Samples underwent PD-L1/2 immunostaining, tumour mutational burden (TMB) evaluation, and high-resolution T-cell receptor (TCR) sequencing to derive T-cell clonality and targeted transcriptomics. (3) Results: We analysed 24 samples from matched primary (
Identifiants
pubmed: 33946676
pii: cancers13092137
doi: 10.3390/cancers13092137
pmc: PMC8124398
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Wellcome Trust
ID : PS3416
Pays : United Kingdom
Organisme : American Society of Clinical Oncology
ID : 14704
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