The Zinc Finger Antiviral Protein ZAP Restricts Human Cytomegalovirus and Selectively Binds and Destabilizes Viral
DNA virus
HCMV
ISG
ZAP
ZC3HAV1
antiviral
herpesvirus
human cytomegalovirus
innate immunity
interferons
mRNA degradation
pattern recognition receptors
zinc finger proteins
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
04 05 2021
04 05 2021
Historique:
entrez:
5
5
2021
pubmed:
6
5
2021
medline:
14
10
2021
Statut:
epublish
Résumé
Interferon-stimulated gene products (ISGs) play a crucial role in early infection control. The ISG zinc finger CCCH-type antiviral protein 1 (ZAP/ZC3HAV1) antagonizes several RNA viruses by binding to CG-rich RNA sequences, whereas its effect on DNA viruses is less well understood. Here, we decipher the role of ZAP in the context of human cytomegalovirus (HCMV) infection, a β-herpesvirus that is associated with high morbidity in immunosuppressed individuals and newborns. We show that expression of the two major isoforms of ZAP, ZAP-S and ZAP-L, is induced during HCMV infection and that both negatively affect HCMV replication. Transcriptome and proteome analyses demonstrated that the expression of ZAP results in reduced viral mRNA and protein levels and decelerates the progression of HCMV infection. Metabolic RNA labeling combined with high-throughput sequencing (SLAM-seq) revealed that most of the gene expression changes late in infection result from the general attenuation of HCMV. Furthermore, at early stages of infection, ZAP restricts HCMV by destabilizing a distinct subset of viral mRNAs, particularly those from the previously uncharacterized
Identifiants
pubmed: 33947766
pii: mBio.02683-20
doi: 10.1128/mBio.02683-20
pmc: PMC8263000
pii:
doi:
Substances chimiques
Protein Isoforms
0
RNA-Binding Proteins
0
UL4 protein, Human cytomegalovirus
0
Viral Envelope Proteins
0
Viral Proteins
0
ZC3HAV1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 Gonzalez-Perez et al.
Références
J Infect Dis. 1986 Mar;153(3):478-88
pubmed: 3005424
PLoS Pathog. 2017 Jan 6;13(1):e1006145
pubmed: 28060952
Nat Protoc. 2016 Dec;11(12):2301-2319
pubmed: 27809316
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
J Virol. 1978 Jun;26(3):686-701
pubmed: 209215
Nature. 2011 Apr 28;472(7344):481-5
pubmed: 21478870
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
Acta Biochim Pol. 2015;62(1):97-101
pubmed: 25730208
J Virol. 2003 Nov;77(21):11555-62
pubmed: 14557641
Bioinformatics. 2015 Apr 1;31(7):1130-2
pubmed: 25417205
Genome Biol. 2016 Jul 05;17(1):148
pubmed: 27380939
Nat Immunol. 2019 Apr;20(4):493-502
pubmed: 30833792
J Virol. 1982 Feb;41(2):462-77
pubmed: 6281461
Nat Commun. 2014 Nov 10;5:5362
pubmed: 25382312
Nucleic Acids Res. 2016 Jul 8;44(W1):W160-5
pubmed: 27079975
J Virol. 2002 Sep;76(17):8596-608
pubmed: 12163579
J Virol. 2004 Dec;78(23):12781-7
pubmed: 15542630
Cell. 2014 Jun 5;157(6):1460-1472
pubmed: 24906157
Nat Methods. 2017 Dec;14(12):1198-1204
pubmed: 28945705
Nucleic Acids Res. 2012 May;40(10):4288-97
pubmed: 22287627
J Virol. 1989 Jan;63(1):281-90
pubmed: 2535734
Nat Methods. 2016 Jun;13(6):508-14
pubmed: 27018577
PLoS Genet. 2008 Jan;4(1):e21
pubmed: 18225958
Nat Immunol. 2019 Dec;20(12):1610-1620
pubmed: 31740798
Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11085-90
pubmed: 23776219
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
J Virol. 2007 Oct;81(20):11267-81
pubmed: 17686857
Med Microbiol Immunol. 2019 Aug;208(3-4):495-512
pubmed: 30805724
Nature. 2017 Oct 5;550(7674):124-127
pubmed: 28953888
Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15834-9
pubmed: 21876179
Arch Dis Child. 1991 Sep;66(9):1068-9
pubmed: 1656894
J Gen Virol. 2008 Feb;89(Pt 2):359-368
pubmed: 18198366
Proc Natl Acad Sci U S A. 2015 Nov 10;112(45):14048-53
pubmed: 26504237
Curr Top Microbiol Immunol. 1990;154:125-69
pubmed: 2161319
J Med Virol. 2012 Jun;84(6):890-6
pubmed: 22499011
J Virol Methods. 1989 Feb;23(2):157-67
pubmed: 2542350
Am J Transplant. 2004 Aug;4(8):1219-26
pubmed: 15268722
Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):151-6
pubmed: 17185417
Bioinformatics. 2011 Jun 15;27(12):1653-9
pubmed: 21543442
Nat Biotechnol. 2014 Sep;32(9):941-6
pubmed: 24952903
Virol J. 2015 Dec 01;12:203
pubmed: 26625984
Nucleic Acids Res. 2019 Sep 5;47(15):8061-8083
pubmed: 31276592
Medicine (Baltimore). 1985 Mar;64(2):100-14
pubmed: 2983175
PLoS Pathog. 2020 Sep 4;16(9):e1008844
pubmed: 32886716
Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24303-24309
pubmed: 31719195
Int J Genomics. 2016;2016:9543274
pubmed: 27314006
PLoS Pathog. 2020 Aug 31;16(8):e1008845
pubmed: 32866210
J Virol. 2019 Aug 28;93(18):
pubmed: 31118263
Elife. 2019 Dec 24;8:
pubmed: 31873071
Oncogene. 2014 Aug 28;33(35):4407-17
pubmed: 24469038
J Virol. 2017 Apr 13;91(9):
pubmed: 28202764
Gene. 1991 May 30;101(2):209-13
pubmed: 1647360
PLoS Pathog. 2013;9(5):e1003366
pubmed: 23717203
PLoS Pathog. 2011 Dec;7(12):e1002444
pubmed: 22241980
Nature. 2014 Jan 30;505(7485):691-5
pubmed: 24284630
J Gen Virol. 2003 Jan;84(Pt 1):17-28
pubmed: 12533697
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11995-9
pubmed: 7505451
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Virology. 2020 Jan 15;540:57-65
pubmed: 31739185
Bioinformatics. 2018 Dec 1;34(23):4054-4063
pubmed: 29931226
J Gen Virol. 2018 Jan;99(1):73-85
pubmed: 29182509
Int J Oncol. 2003 Aug;23(2):541-7
pubmed: 12851707
J Virol. 2001 Nov;75(22):11218-21
pubmed: 11602761
Protein Cell. 2010 Aug;1(8):752-9
pubmed: 21203916
Bioinformatics. 2018 Jul 1;34(13):i218-i226
pubmed: 29949974
J Virol. 2007 Mar;81(5):2391-400
pubmed: 17182693
Curr Biol. 1993 Jun 1;3(6):384-6
pubmed: 15335739
Semin Immunopathol. 2014 Nov;36(6):627-40
pubmed: 25288477
mBio. 2018 Mar 13;9(2):
pubmed: 29535194
Elife. 2019 Jul 09;8:
pubmed: 31284899
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
PLoS One. 2012;7(11):e50166
pubmed: 23226246
Front Microbiol. 2020 Feb 07;11:124
pubmed: 32117146
PLoS Pathog. 2018 Jul 17;14(7):e1007166
pubmed: 30016363
J Virol. 2017 Jan 3;91(2):
pubmed: 27807230
J Gen Virol. 2003 Mar;84(Pt 3):657-663
pubmed: 12604818
Trends Biochem Sci. 2001 May;26(5):273-5
pubmed: 11343911
PLoS Pathog. 2013;9(7):e1003494
pubmed: 23853601
Blood. 2007 Aug 1;110(3):937-45
pubmed: 17440050
Cell Rep. 2020 Jan 7;30(1):46-52.e4
pubmed: 31914396
Annu Rev Immunol. 2014;32:513-45
pubmed: 24555472
J Virol. 1989 Aug;63(8):3330-7
pubmed: 2545908
RNA Biol. 2008 Apr-Jun;5(2):65-7
pubmed: 18418085