Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes.
Animals
Antibodies, Monoclonal
/ blood
Antibodies, Neutralizing
/ blood
Antibodies, Viral
/ blood
Antibody Affinity
COVID-19
/ immunology
Epitopes
/ immunology
Humans
Immune Evasion
Immunoglobulin G
/ blood
Immunoglobulin Heavy Chains
/ immunology
Immunoglobulin Variable Region
/ immunology
Mice
Mice, Inbred BALB C
Mutation
Protein Domains
Proteomics
SARS-CoV-2
/ genetics
Spike Glycoprotein, Coronavirus
/ chemistry
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
04 06 2021
04 06 2021
Historique:
received:
12
01
2021
accepted:
29
04
2021
pubmed:
6
5
2021
medline:
12
6
2021
entrez:
5
5
2021
Statut:
ppublish
Résumé
The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are unknown. Proteomic deconvolution of the IgG repertoire to the spike glycoprotein in convalescent subjects revealed that the response is directed predominantly (>80%) against epitopes residing outside the receptor binding domain (RBD). In one subject, just four IgG lineages accounted for 93.5% of the response, including an amino (N)-terminal domain (NTD)-directed antibody that was protective against lethal viral challenge. Genetic, structural, and functional characterization of a multidonor class of "public" antibodies revealed an NTD epitope that is recurrently mutated among emerging SARS-CoV-2 variants of concern. These data show that "public" NTD-directed and other non-RBD plasma antibodies are prevalent and have implications for SARS-CoV-2 protection and antibody escape.
Identifiants
pubmed: 33947773
pii: science.abg5268
doi: 10.1126/science.abg5268
pmc: PMC8224265
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antibodies, Neutralizing
0
Antibodies, Viral
0
Epitopes
0
Immunoglobulin G
0
Immunoglobulin Heavy Chains
0
Immunoglobulin Variable Region
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1108-1112Subventions
Organisme : NIAID NIH HHS
ID : 75N93019C00050
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103311
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI127521
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA260543
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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