ImmunoPET of the differential expression of CD146 in breast cancer.
52Mn
89Zr
CD146
ImmunoPET
YY146
breast cancer
optical imaging
Journal
American journal of cancer research
ISSN: 2156-6976
Titre abrégé: Am J Cancer Res
Pays: United States
ID NLM: 101549944
Informations de publication
Date de publication:
2021
2021
Historique:
received:
09
12
2020
accepted:
20
01
2021
entrez:
5
5
2021
pubmed:
6
5
2021
medline:
6
5
2021
Statut:
epublish
Résumé
With advancement in antibody engineering, the development and characterization of new cancer-specific molecular targets are in the forefront of this PET-antibody combination "revolution". Overexpression of CD146 in different types of tumors, including breast tumor, has been associated with tumor progression and poor prognosis. Non-invasive detection of CD146 with a monoclonal antibody may provide a noninvasive diagnostic tool with high specificity and accountability. Herein, we have developed a CD146-specific monoclonal antibody (YY146), radiolabeled it with Western Blot results show that MDA-MB-435 cell line had greater levels of CD146 expression when compared to the other cell lines investigated. Flow cytometry confirmed binding ability of YY146. PET images revealed well correlated uptake between tumor uptake and CD146 expression levels, confirmed by biodistribution studies and fluorescence imaging. PET imaging, for up to 7 days, of mice bearing three different breast tumors were carried out and revealed radiotracer uptake in tumors that strongly (r
Types de publication
Journal Article
Langues
eng
Pagination
1586-1599Informations de copyright
AJCR Copyright © 2021.
Déclaration de conflit d'intérêts
None.
Références
Breast Dis. 2010;32(1-2):35-48
pubmed: 21778573
Future Oncol. 2009 Oct;5(8):1129-43
pubmed: 19852726
Int J Environ Res Public Health. 2020 Mar 20;17(6):
pubmed: 32245065
Int J Womens Health. 2019 Jul 31;11:431-437
pubmed: 31447592
Cancer Immun. 2012;12:14
pubmed: 22896759
Appl Radiat Isot. 2019 Apr;146:99-103
pubmed: 30763821
Proc Natl Acad Sci U S A. 1989 Dec;86(24):9891-5
pubmed: 2602381
Am J Nucl Med Mol Imaging. 2020 Dec 15;10(6):279-292
pubmed: 33329930
J Clin Oncol. 2005 Oct 10;23(29):7350-60
pubmed: 16145060
Breast Cancer Res. 2009;11(1):R1
pubmed: 19123925
Am J Cancer Res. 2020 Jan 01;10(1):211-223
pubmed: 32064162
Breast Cancer (Dove Med Press). 2019 Apr 10;11:151-164
pubmed: 31040712
Crit Rev Oncol Hematol. 2020 Jan;145:102855
pubmed: 31927455
Mol Pharm. 2017 Oct 2;14(10):3239-3247
pubmed: 28825843
Am J Nucl Med Mol Imaging. 2019 Feb 15;9(1):12-23
pubmed: 30911434
Histochem Cell Biol. 2008 Apr;129(4):441-51
pubmed: 18214516
Nat Rev Clin Oncol. 2016 Nov;13(11):674-690
pubmed: 27184417
Nucl Med Biol. 2013 Jan;40(1):3-14
pubmed: 22998840
Cancers (Basel). 2018 May 05;10(5):
pubmed: 29734758
Am J Nucl Med Mol Imaging. 2019 Feb 15;9(1):30-66
pubmed: 30911436
J Photochem Photobiol B. 2010 Jan 21;98(1):77-94
pubmed: 20031443
Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):E6525-34
pubmed: 26553993
J Vis Exp. 2019 Jan 20;(143):
pubmed: 30735150
Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1127-32
pubmed: 22210108
J Proteomics. 2014 May 30;103:153-69
pubmed: 24704855
Mol Pharm. 2016 Jul 5;13(7):2563-70
pubmed: 27280694
Theranostics. 2016 Aug 08;6(11):1918-33
pubmed: 27570560
Clin Breast Cancer. 2009 Jun;9 Suppl 2:S73-81
pubmed: 19596646
Hum Pathol. 2007 Jun;38(6):830-41
pubmed: 17316758
Am J Cancer Res. 2020 Jan 01;10(1):224-236
pubmed: 32064163
Hum Pathol. 2015 Sep;46(9):1267-74
pubmed: 26170011
Am J Cancer Res. 2020 Jan 01;10(1):95-113
pubmed: 32064155
Blood. 2006 Oct 15;108(8):2578-86
pubmed: 16825498
Eur J Nucl Med Mol Imaging. 2011 Nov;38(11):2066-76
pubmed: 21814852
Bioconjug Chem. 2015 Oct 21;26(10):2118-24
pubmed: 26317429