Silver Staining of 2D Electrophoresis Gels.
Mass spectrometry
Polyacrylamide gels
Protein visualization
Quantification
Silver staining
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
5
5
2021
pubmed:
6
5
2021
medline:
24
6
2021
Statut:
ppublish
Résumé
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and quantitative response. Both features are discussed in this chapter, and optimized silver staining protocols are proposed.
Identifiants
pubmed: 33950484
doi: 10.1007/978-1-0716-1024-4_6
doi:
Substances chimiques
Proteins
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
63-75Références
Switzer RC, Merril CR, Shifrin S (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem 98:231–237
doi: 10.1016/0003-2697(79)90732-2
Rabilloud T (1990) Mechanisms of protein silver staining in polyacrylamide gels: a 10-year synthesis. Electrophoresis 11:785–794
doi: 10.1002/elps.1150111003
Rabilloud T, Vuillard L, Gilly C et al (1994) Silver-staining of proteins in polyacrylamide gels: a general overview. Cell Mol Biol 40:57–75
pubmed: 8003936
Blum H, Beier H, Gross HJ (1987) The expression of the TMV-specific 30-kDa protein in tobacco protoplasts is strongly and selectively enhanced by actinomycin. Electrophoresis 8:93–99
doi: 10.1002/elps.1150080203
Richert S, Luche S, Chevallet M et al (2004) About the mechanism of interference of silver staining with peptide mass spectrometry. Proteomics 4:909–916
doi: 10.1002/pmic.200300642
Metz B, Kersten GF, Hoogerhout P et al (2004) Identification of formaldehyde-induced modifications in proteins: reactions with model peptides. J Biol Chem 279:6235–6243
doi: 10.1074/jbc.M310752200
Metz B, Kersten GF, Baart GJ et al (2006) Identification of formaldehyde-induced modifications in proteins: reactions with insulin. Bioconjug Chem 17:815–822
doi: 10.1021/bc050340f
Oses-Prieto JA, Zhan X, Burlingame AL (2007) Formation of epsilon-formyllysine on silver-stained proteins: implications for assignment of isobaric dimethylation sites by tandem mass spectrometry. MCP 6:181–192
pubmed: 17138591
Gharahdaghi F, Weinberg CR, Meagher DA et al (1999) Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: a method for the removal of silver ions to enhance sensitivity. Electrophoresis 20:601–605
doi: 10.1002/(SICI)1522-2683(19990301)20:3<601::AID-ELPS601>3.0.CO;2-6
Chevallet M, Luche S, Diemer H et al (2008) Sweet silver: a formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry. Proteomics 8:4853–4861
doi: 10.1002/pmic.200800321
Sinha P, Poland J, Schnolzer M et al (2001) A new silver staining apparatus and procedure for matrix-assisted laser desorption/ionization-time of flight analysis of proteins after two-dimensional electrophoresis. Proteomics 1:835–840
doi: 10.1002/1615-9861(200107)1:7<835::AID-PROT835>3.0.CO;2-2
Rabilloud T (1992) A comparison between low background silver diammine and silver nitrate protein stains. Electrophoresis 13:429–439
doi: 10.1002/elps.1150130190
Chevallet M, Luche S, Rabilloud T (2006) Silver staining of proteins in polyacrylamide gels. Nat Protoc 1:1852–1858
doi: 10.1038/nprot.2006.288
Hochstrasser DF, Merril CR (1988) ‘Catalysts’ for polyacrylamide gel polymerization and detection of proteins by silver staining. Appl Theor Electrophor 1:35–40
pubmed: 2484987
Shevchenko A, Wilm M, Vorm O et al (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68:850–858
doi: 10.1021/ac950914h