Number and Replating Capacity of Endothelial Colony-Forming Cells are Telomere Length Dependent: Implication for Human Atherogenesis.
Adult
Aged
Aged, 80 and over
Aging
/ metabolism
Atherosclerosis
/ metabolism
Cell Proliferation
Cells, Cultured
Endothelial Progenitor Cells
/ metabolism
Female
Humans
Male
Middle Aged
Neovascularization, Physiologic
/ physiology
Retrospective Studies
Telomere
/ metabolism
Telomere Homeostasis
/ physiology
Young Adult
aging
endothelial progenitor cell
telomere
Journal
Journal of the American Heart Association
ISSN: 2047-9980
Titre abrégé: J Am Heart Assoc
Pays: England
ID NLM: 101580524
Informations de publication
Date de publication:
18 05 2021
18 05 2021
Historique:
pubmed:
7
5
2021
medline:
28
10
2021
entrez:
6
5
2021
Statut:
ppublish
Résumé
Background Short leukocyte telomere length (TL) is associated with atherosclerotic cardiovascular disease. Endothelial repair plays a key role in the development of atherosclerosis. The objective was to examine associations between TL and proliferative dynamics of endothelial colony-forming cells (ECFCs), which behave as progenitor cells displaying endothelial repair activity. Methods and Results To isolate ECFCs, we performed a clonogenic assay on blood samples from 116 participants (aged 24-94 years) in the TELARTA (Telomere in Arterial Aging) cohort study. We detected no ECFC clones in 29 (group 1), clones with no replating capacity in other 29 (group 2), and clones with replating capacity in the additional 58 (group 3). Leukocyte TL was measured by Southern blotting and ECFCs (ECFC-TL). Age- and sex-adjusted leukocyte TL (mean±SEM) was the shortest in group 1 (6.51±0.13 kb), longer in group 2 (6.69±0.13 kb), and the longest in group 3 (6.78±0.09 kb) (
Identifiants
pubmed: 33955230
doi: 10.1161/JAHA.120.020606
pmc: PMC8200696
doi:
Banques de données
ClinicalTrials.gov
['NCT02176941']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e020606Subventions
Organisme : NICHD NIH HHS
ID : R01 HD071180
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL116446
Pays : United States
Investigateurs
Sylvie Gautier
(S)
Ghassan Watfa
(G)
Huguette Louis
(H)
Mélanie Folio
(M)
Margaux Schmitt
(M)
Oualid Ayad
(O)
Agnes Didier
(A)
Sandrine Vauthier
(S)
Nelly François
(N)
Sophie Visvikis-Siest
(S)
Maria G Stathopolou
(MG)
Masayuki Kimura
(M)
Pascal M Rossi
(PM)
Patricia Béranger
(P)
Serguei Malikov
(S)
Nicla Settembre
(N)
Jacques Hubert
(J)
Luc Frimat
(L)
Baptiste Bertrand
(B)
Mourad Boufi
(M)
Xavier Flecher
(X)
Nicolas Sadoul
(N)
Pascal Eschwege
(P)
Michèle Kessler
(M)
Irene P Tzanetakou
(IP)
Ilias P Doulamis
(IP)
Panagiotis Konstantopoulos
(P)
Aspasia Tzani
(A)
Marilina Korou
(M)
Anastasios Gkogkos
(A)
Konstantinos Perreas
(K)
Evangelos Menenakos
(E)
Georgios Samanidis
(G)
Michail Vasiloglou-Gkanis
(M)
Jeremy D Kark
(JD)
Simon Verhulst
(S)
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