Naive stem cell blastocyst model captures human embryo lineage segregation.
blastocyst
embryonic stem cells
epiblast
human embryo
hypoblast
lineage segregation
pluripotency
self-organization
trophoblast
Journal
Cell stem cell
ISSN: 1875-9777
Titre abrégé: Cell Stem Cell
Pays: United States
ID NLM: 101311472
Informations de publication
Date de publication:
03 06 2021
03 06 2021
Historique:
received:
09
04
2021
revised:
27
04
2021
accepted:
28
04
2021
pubmed:
7
5
2021
medline:
25
6
2021
entrez:
6
5
2021
Statut:
ppublish
Résumé
Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research.
Identifiants
pubmed: 33957081
pii: S1934-5909(21)00207-1
doi: 10.1016/j.stem.2021.04.031
pmc: PMC8189436
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1016-1022.e4Subventions
Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203151/Z/16/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1100526
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P010423/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1100526/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P00072X/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_12009
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P00072X/2
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests G.G. and A.S. are inventors on a patent relating to human naive pluripotent stem cells filed by the University of Cambridge.
Références
Bioinformatics. 2014 Apr 1;30(7):923-30
pubmed: 24227677
Stem Cell Reports. 2021 May 11;16(5):1031-1038
pubmed: 33667412
Dev Cell. 2019 Dec 16;51(6):698-712.e8
pubmed: 31846649
Development. 2018 Nov 9;145(21):
pubmed: 30413530
Stem Cell Reports. 2015 Dec 8;5(6):954-962
pubmed: 26626176
Stem Cell Reports. 2016 Apr 12;6(4):437-446
pubmed: 26947977
Nat Methods. 2019 Dec;16(12):1289-1296
pubmed: 31740819
Cell. 2016 May 5;165(4):1012-26
pubmed: 27062923
Cell. 2014 Sep 11;158(6):1254-1269
pubmed: 25215486
Nature. 2021 Mar;591(7851):627-632
pubmed: 33731926
Development. 2018 Feb 7;145(3):
pubmed: 29361568
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Stem Cell Reports. 2019 Jun 11;12(6):1212-1222
pubmed: 31031191
Stem Cell Reports. 2019 Dec 10;13(6):1083-1098
pubmed: 31708477
Development. 2015 Sep 15;142(18):3151-65
pubmed: 26293300
Dev Biol. 2012 Jan 15;361(2):358-63
pubmed: 22079695
Cell Stem Cell. 2021 Jun 3;28(6):1023-1039.e13
pubmed: 33831365
Development. 2017 Aug 1;144(15):2748-2763
pubmed: 28765214
Development. 2019 Apr 3;146(7):
pubmed: 30944104
Dev Cell. 2015 Nov 9;35(3):366-82
pubmed: 26555056
Nature. 2018 May;557(7703):106-111
pubmed: 29720634
Nat Methods. 2013 Nov;10(11):1096-8
pubmed: 24056875
Nat Cell Biol. 2021 Jan;23(1):49-60
pubmed: 33420491
Nat Cell Biol. 2016 Jun;18(6):700-708
pubmed: 27144686
Cell Stem Cell. 2021 Jun 3;28(6):1040-1056.e6
pubmed: 33831366
Nature. 2021 Mar;591(7851):620-626
pubmed: 33731924
Dev Biol. 2013 Mar 1;375(1):54-64
pubmed: 23261930
Nature. 2019 Sep;573(7774):421-425
pubmed: 31511693
Nat Cell Biol. 2018 May;20(5):553-564
pubmed: 29695788
Annu Rev Genet. 2018 Nov 23;52:185-201
pubmed: 30183407
Cell. 2014 Feb 27;156(5):1032-44
pubmed: 24529478
Nature. 2016 May 04;533(7602):251-4
pubmed: 27144363
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886