Protocol for a prospective, observational, deep phenotyping study on adipose epigenetic and lipidomic determinants of metabolic homoeostasis in South Asian Indians: the Indian Diabetes and Metabolic Health (InDiMeT) study.
biochemistry
diabetes & endocrinology
genetics
molecular biology
physiology
Journal
BMJ open
ISSN: 2044-6055
Titre abrégé: BMJ Open
Pays: England
ID NLM: 101552874
Informations de publication
Date de publication:
06 05 2021
06 05 2021
Historique:
entrez:
7
5
2021
pubmed:
8
5
2021
medline:
5
6
2021
Statut:
epublish
Résumé
We describe the rationale and broad study design of the Indian Diabetes and Metabolic Health (InDiMeT) study, a new prospective, observational study incorporating extensive epigenetic (DNA methylation) and lipidomic signatures to examine their association with the dysregulation of adipose de novo lipogenesis (DNL) in South Asian Indians. The InDiMeT study aims to use a case-control design to identify genetic and modifiable-environmental-lifestyle associated determinants of (1) epigenomic (DNA methylome) dysregulation of adipose DNL in type 2 diabetes mellitus (T2DM) adipose tissue, (2) identify correlates of epigenomic (DNA methylome) dysregulation of adipose DNL in peripheral blood mononuclear cells (PBMCs) from T2DM subjects and (3) elucidate plasma lipidomic correlates of adipose DNL in T2DM that can be used as biomarkers of adipose tissue dysfunction. The InDiMeT study will involve recruitment of 176 normoglycaemic and T2DM individuals who will be undergoing laparoscopic surgery for clinical conditions. Extensive phenotyping of the subjects will be conducted and DNA methylome and lipidomic measurements will be made. The adipose DNL pathway genes are likely to be hypermethylated in patients with T2DM with corresponding reduction of gene expression. Correlates of epigenomic (DNA methylome) dysregulation of adipose DNL pathway in PBMCs and their adipose and plasma lipidomic signatures in T2DM subjects could act as early markers of development of T2DM. For the InDiMeT study, ethical approval for addressing the specific aims has been obtained from the Institutional Ethics Committee, St John's Medical College and Hospital, St John's National Academy of Health Sciences, Bangalore. Findings from this study will be disseminated through scientific publications in peer-reviewed journals, research conferences and via presentations to stakeholders, patients, clinicians, public and policymakers through appropriate channels.
Identifiants
pubmed: 33958336
pii: bmjopen-2020-043644
doi: 10.1136/bmjopen-2020-043644
pmc: PMC8103950
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e043644Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust/DBT India Alliance
ID : IA/CPHI/19/1/504593
Informations de copyright
© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: None declared.
Références
BMC Bioinformatics. 2009 Jul 27;10:232
pubmed: 19635165
Diabetes. 2014 Sep;63(9):2962-76
pubmed: 24812430
Nutrients. 2018 Sep 29;10(10):
pubmed: 30274245
Diabetologia. 2009 May;52(5):882-90
pubmed: 19252892
Indian J Public Health. 2012 Jan-Mar;56(1):103-4
pubmed: 22684186
PLoS Genet. 2012;8(8):e1002793
pubmed: 22876189
Nat Commun. 2016 Nov 25;7:13507
pubmed: 27886173
Am J Physiol Endocrinol Metab. 2004 Apr;286(4):E577-88
pubmed: 14600072
Diabetologia. 1985 Jul;28(7):412-9
pubmed: 3899825
Hum Mol Genet. 2018 May 15;27(10):1830-1846
pubmed: 29566149
Nat Genet. 2012 Sep;44(9):981-90
pubmed: 22885922
Diabetes. 2013 Jan;62(1):1-8
pubmed: 23258903
Cell. 2014 Oct 9;159(2):318-32
pubmed: 25303528
Epigenetics. 2015;10(4):274-81
pubmed: 25634226
Metabolism. 2006 Dec;55(12):1569-73
pubmed: 17142126
Nature. 2014 Jun 5;510(7503):84-91
pubmed: 24899308
Br J Nutr. 2009 Nov;102(10):1498-506
pubmed: 19586573
Diabetes Care. 2012 Mar;35(3):605-11
pubmed: 22266733
Nat Biotechnol. 2010 Oct;28(10):1097-105
pubmed: 20852635
Am J Clin Nutr. 1987 Jan;45(1):78-85
pubmed: 3799507
Am J Clin Nutr. 2015 Jan;101(1):153-63
pubmed: 25527759
Crit Rev Biochem Mol Biol. 2010 Jun;45(3):199-214
pubmed: 20218765
Brief Bioinform. 2015 May;16(3):369-79
pubmed: 24867940
Am J Clin Nutr. 2015 Jan;101(1):34-43
pubmed: 25527748
Nucleic Acids Res. 2005 Oct 13;33(18):5868-77
pubmed: 16224102
Am J Physiol Endocrinol Metab. 2005 Oct;289(4):E551-61
pubmed: 15928024
Diabetologia. 2016 Dec;59(12):2664-2673
pubmed: 27627980
BMC Public Health. 2012 Aug 28;12:701
pubmed: 22928740
Diabetes Care. 2004 Jun;27(6):1487-95
pubmed: 15161807
J Biol Chem. 1993 Oct 25;268(30):22243-6
pubmed: 8226728
Public Health Nutr. 2010 Jan;13(1):47-53
pubmed: 19656418
Asia Pac J Clin Nutr. 2008;17(1):178-85
pubmed: 18364343
Glob Health Epidemiol Genom. 2017 Feb 14;2:e2
pubmed: 29868214
Diabetes. 2016 Jul;65(7):1800-7
pubmed: 27288005