Neuron Replating, a Powerful and Versatile Approach to Study Early Aspects of Neuron Differentiation.
actin
cofilin
growth
neurite
neuron replating
replating
Journal
eNeuro
ISSN: 2373-2822
Titre abrégé: eNeuro
Pays: United States
ID NLM: 101647362
Informations de publication
Date de publication:
Historique:
received:
27
11
2020
revised:
12
04
2021
accepted:
20
04
2021
pubmed:
8
5
2021
medline:
3
7
2021
entrez:
7
5
2021
Statut:
epublish
Résumé
Neuron differentiation includes formation and outgrowth of neurites that differentiate into axons or dendrites. Directed neurite outgrowth is controlled by growth cones that protrude and retract actin-rich structures to sense environmental cues. These cues control local actin filament dynamics, steer growth cones toward attractants and away from repellents, and navigate neurites through the developing brain. Rodent hippocampal neurons are widely used to study the mechanisms underlying neuron differentiation. Genetic manipulation of isolated neurons including gene inactivation or reporter gene expression can be achieved by classical transfections methods, but these methods are restricted to neurons cultured for several days, after neurite formation or outgrowth. Instead, electroporation allows gene manipulation before seeding. However, reporter gene expression usually takes up to 24 h, and time course of gene inactivation depends on the half live of the targeted mRNA and gene product. Hence, these methods do not allow to study early aspects of neuron differentiation. In the present study, we provide a detailed protocol in which we combined electroporation-based gene manipulation of mouse hippocampal neurons before initial seeding with a replating step after 2 d
Identifiants
pubmed: 33958372
pii: ENEURO.0536-20.2021
doi: 10.1523/ENEURO.0536-20.2021
pmc: PMC8143016
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 Schneider et al.
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