Utility of red-light ultrafast optogenetic stimulation of the auditory pathway.
channelrhodopsin
cochlear implant
dynamic range
gating
spiral ganglion
temporal coding
Journal
EMBO molecular medicine
ISSN: 1757-4684
Titre abrégé: EMBO Mol Med
Pays: England
ID NLM: 101487380
Informations de publication
Date de publication:
07 06 2021
07 06 2021
Historique:
revised:
22
03
2021
received:
04
09
2020
accepted:
23
03
2021
pubmed:
8
5
2021
medline:
26
10
2021
entrez:
7
5
2021
Statut:
ppublish
Résumé
Optogenetic stimulation of spiral ganglion neurons (SGNs) in the ear provides a future alternative to electrical stimulation used in current cochlear implants. Here, we employed fast and very fast variants of the red-light-activated channelrhodopsin (ChR) Chrimson (f-Chrimson and vf-Chrimson) to study their utility for optogenetic stimulation of SGNs in mice. The light requirements were higher for vf-Chrimson than for f-Chrimson, even when optimizing membrane expression of vf-Chrimson by adding potassium channel trafficking sequences. Optogenetic time and intensity coding by single putative SGNs were compared with coding of acoustic clicks. vf-Chrimson enabled putative SGNs to fire at near-physiological rates with good temporal precision up to 250 Hz of stimulation. The dynamic range of SGN spike rate coding upon optogenetic stimulation was narrower than for acoustic clicks but larger than reported for electrical stimulation. The dynamic range of spike timing, on the other hand, was more comparable for optogenetic and acoustic stimulation. In conclusion, f-Chrimson and vf-Chrimson are promising candidates for optogenetic stimulation of SGNs in auditory research and future cochlear implants.
Identifiants
pubmed: 33960685
doi: 10.15252/emmm.202013391
pmc: PMC8185542
doi:
Substances chimiques
Channelrhodopsins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13391Informations de copyright
© 2021 The Authors. Published under the terms of the CC BY 4.0 license.
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