Soybean extract modified by Aspergillus awamori stimulates a greater collagen-I synthesis in the intracellular matrix of human fibroblasts.
Daidzein
Genistein
biotransformation
collagen
fibroblasts
isoflavones
soybean
Journal
Journal of cosmetic dermatology
ISSN: 1473-2165
Titre abrégé: J Cosmet Dermatol
Pays: England
ID NLM: 101130964
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
08
04
2021
received:
08
02
2021
accepted:
30
04
2021
pubmed:
11
5
2021
medline:
1
3
2022
entrez:
10
5
2021
Statut:
ppublish
Résumé
Aglycone isoflavones are estrogen-like bioactive compounds found in low amounts in soybean, which are increased by biotransformation processes. This study investigated two biotransformation processes of soybean extracts with Aspergillus awamori fungus, evaluating aglycone content and capability of stimulation of collagen-I deposition. Isoflavones were quantified via HPLC; cytotoxicity of biotransformed extracts toward mouse and human fibroblasts was evaluated via NRU and apoptosis/necrosis assays; and collagen-I deposition was measured through Western blot, immunofluorescence, and immunoassay. BSE-2 was the biotransformed soybean extract with the highest aglycone content and did not decrease viability or demonstrated cytotoxicity to either L929 or HDFa cells. BSE-2, at the optimal concentration of 1.33 μg/mL, increased substantially collagen-I amount in HDFa intracellular matrix compared to non-biotransformed soybean extract (NBSE) and immunoassay demonstrated that the extracellular deposition was mostly inhibited by BSE-2 concentrations, except at 1.33 μg/mL. Hence, biotransformed soybean extract by the enzymatic filtrate of Aspergillus awamori fungus demonstrated a high nutricosmetic potential, showing safeness and effective collagen-I augmentation.
Substances chimiques
Collagen Type I
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1243-1250Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2012/04225-4
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2012/23271-7
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 168880/2018-0
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 88882.180867/2018-01
Informations de copyright
© 2021 Wiley Periodicals LLC.
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