Host factor Rab11a is critical for efficient assembly of influenza A virus genomic segments.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
23
03
2021
accepted:
19
04
2021
revised:
20
05
2021
pubmed:
11
5
2021
medline:
5
10
2021
entrez:
10
5
2021
Statut:
epublish
Résumé
It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion; however, the role of host factors in genome assembly is not completely understood. To evaluate the significance of cellular factors in genome assembly, we generated a reporter virus carrying a tetracysteine tag in the NP gene (NP-Tc virus) and assessed the dynamics of vRNP localization with cellular components by fluorescence microscopy. At early time points, vRNP complexes were preferentially exported to the MTOC; subsequently, vRNPs associated on vesicles positive for cellular factor Rab11a and formed distinct vRNP bundles that trafficked to the plasma membrane on microtubule networks. In Rab11a deficient cells, however, vRNP bundles were smaller in the cytoplasm with less co-localization between different vRNP segments. Furthermore, Rab11a deficiency increased the production of non-infectious particles with higher RNA copy number to PFU ratios, indicative of defects in specific genome assembly. These results indicate that Rab11a+ vesicles serve as hubs for the congregation of vRNP complexes and enable specific genome assembly through vRNP:vRNP interactions, revealing the importance of Rab11a as a critical host factor for influenza A virus genome assembly.
Identifiants
pubmed: 33970958
doi: 10.1371/journal.ppat.1009517
pii: PPATHOGENS-D-21-00621
pmc: PMC8136845
doi:
Substances chimiques
Ribonucleoproteins
0
Viral Proteins
0
rab11 protein
EC 3.6.1.-
rab GTP-Binding Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009517Subventions
Organisme : NIAID NIH HHS
ID : R01 AI123359
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM137961
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI127775
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM066522
Pays : United States
Organisme : NIAID NIH HHS
ID : HHSN272201400004C
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007183
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI125268
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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