EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice.
cell biology
cell morphology
developmental biology
exocyst complex
mouse
spermatogenesis
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
11 05 2021
11 05 2021
Historique:
received:
07
06
2020
accepted:
21
04
2021
entrez:
11
5
2021
pubmed:
12
5
2021
medline:
25
2
2023
Statut:
epublish
Résumé
The male germ cells must adopt the correct morphology at each differentiation stage for proper spermatogenesis. The spermatogonia regulates its differentiation state by its own migration. The male germ cells differentiate and mature with the formation of syncytia, failure of forming the appropriate syncytia results in the arrest at the spermatocyte stage. However, the detailed molecular mechanisms of male germ cell morphological regulation are unknown. Here, we found that EXOC1, a member of the Exocyst complex, is important for the pseudopod formation of spermatogonia and spermatocyte syncytia in mice. EXOC1 contributes to the pseudopod formation of spermatogonia by inactivating the Rho family small GTPase Rac1 and also functions in the spermatocyte syncytia with the SNARE proteins STX2 and SNAP23. Since EXOC1 is known to bind to several cell morphogenesis factors, this study is expected to be the starting point for the discovery of many morphological regulators of male germ cells.
Identifiants
pubmed: 33973520
doi: 10.7554/eLife.59759
pii: 59759
pmc: PMC8112867
doi:
pii:
Substances chimiques
Exoc1 protein, mouse
0
Vesicular Transport Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021, Osawa et al.
Déclaration de conflit d'intérêts
YO, KM, MU, YK, HL, NM, TN, YD, KK, HS, YI, AK, KM, YT, TD, KY, ME, SY, ST, SM, FS No competing interests declared
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