Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow-based detection.


Journal

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN: 1435-4373
Titre abrégé: Eur J Clin Microbiol Infect Dis
Pays: Germany
ID NLM: 8804297

Informations de publication

Date de publication:
Nov 2021
Historique:
received: 28 10 2020
accepted: 29 04 2021
pubmed: 12 5 2021
medline: 24 12 2021
entrez: 11 5 2021
Statut: ppublish

Résumé

Carbapenemase-producing organisms (CPOs) pose a serious clinical threat and rapid detection tools are essential to aid in patient management. We developed rapid and simple molecular tests to detect bla

Identifiants

pubmed: 33974185
doi: 10.1007/s10096-021-04267-6
pii: 10.1007/s10096-021-04267-6
doi:

Substances chimiques

Anti-Bacterial Agents 0
Bacterial Proteins 0
DNA Primers 0
Recombinases 0
beta-Lactamases EC 3.5.2.6
carbapenemase EC 3.5.2.6

Types de publication

Evaluation Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2447-2453

Informations de copyright

© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Références

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WHO. WHO publishes list of bacteria for which new antibiotics are urgently needed. 2017 26-04-2021]; Available from: https://www.who.int/news/item/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed .
CDC. Clinicians: Information about CRE. 2019 26-04-2021]; Available from: https://www.cdc.gov/hai/organisms/cre/cre-clinicians.html .
WHO. Guidelines for the prevention and control of carbapenem-resistant Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa in health care facilities. 2017 26-04-2021]; Available from: https://www.who.int/infection-prevention/publications/guidelines-cre/en/ .
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doi: 10.1039/C8AN01621F
James A, Macdonald J (2015) Recombinase polymerase amplification: emergence as a critical molecular technology for rapid, low-resource diagnostics. Expert Rev Mol Diagn 15(11):1475–1489
doi: 10.1586/14737159.2015.1090877
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doi: 10.1371/journal.pone.0112146
Hu C et al (2017) Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and thin film transistor sensors. Biosens Bioelectron 96:281–287
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James AS et al (2018) Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future. Virol J 15(1):75–75
doi: 10.1186/s12985-018-0985-8
Bordin A et al (2019) Evaluation of the SpeeDx Carba (beta) multiplex real-time PCR assay for detection of NDM, KPC, OXA-48-like, IMP-4-like and VIM carbapenemase genes. BMC Infect Dis 19(1):571
doi: 10.1186/s12879-019-4176-z
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Auteurs

Abdulrahman K S Ayfan (AKS)

UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Royal Brisbane and Women's Hospital Campus, Brisbane, Australia.
Faculty of Science, Biochemistry Department, King Abdul-Aziz University (KAU), Jeddah, Saudi Arabia.

Joanne Macdonald (J)

School of Science and Engineering, Genecology Research Centre, University of Sunshine Coast (USC), Sunshine Coast, Australia.

Patrick N A Harris (PNA)

UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Royal Brisbane and Women's Hospital Campus, Brisbane, Australia.
Department of Microbiology, Pathology Queensland, Brisbane, Australia.

Claire Heney (C)

Department of Microbiology, Pathology Queensland, Brisbane, Australia.

David L Paterson (DL)

UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Royal Brisbane and Women's Hospital Campus, Brisbane, Australia.

Ella Trembizki (E)

UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Royal Brisbane and Women's Hospital Campus, Brisbane, Australia.

Claire Y T Wang (CYT)

Infection Management and Prevention Service, Queensland Children's Hospital, Brisbane, Australia.

David M Whiley (DM)

UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Royal Brisbane and Women's Hospital Campus, Brisbane, Australia.
Department of Microbiology, Pathology Queensland, Brisbane, Australia.

Hosam M Zowawi (HM)

UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Royal Brisbane and Women's Hospital Campus, Brisbane, Australia.
College of Medicine, King Saud bin Abdul-Aziz University for Health Science (KSAU-HS), Riyadh, Saudi Arabia.
King Abdullah International Medical Research Centre (KAIMRC), Riyadh, Saudi Arabia.

Adam D Irwin (AD)

UQ Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Royal Brisbane and Women's Hospital Campus, Brisbane, Australia. a.irwin@uq.edu.au.
Infection Management and Prevention Service, Queensland Children's Hospital, Brisbane, Australia. a.irwin@uq.edu.au.

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