A Ran-binding protein facilitates nuclear import of human papillomavirus type 16.
Active Transport, Cell Nucleus
Alphapapillomavirus
/ genetics
Capsid Proteins
/ genetics
Cell Nucleus
/ metabolism
Chromatin
/ genetics
DNA, Viral
/ metabolism
Dyneins
/ genetics
Genome, Viral
/ genetics
Guanine Nucleotide Exchange Factors
/ genetics
Humans
Microtubule-Associated Proteins
/ genetics
Mitosis
Papillomavirus Infections
/ virology
Virus Internalization
alpha Karyopherins
/ genetics
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
23
02
2021
accepted:
23
04
2021
revised:
21
05
2021
pubmed:
12
5
2021
medline:
13
10
2021
entrez:
11
5
2021
Statut:
epublish
Résumé
Human papillomaviruses (HPVs) utilize an atypical mode of nuclear import during cell entry. Residing in the Golgi apparatus until mitosis onset, a subviral complex composed of the minor capsid protein L2 and viral DNA (L2/vDNA) is imported into the nucleus after nuclear envelope breakdown by associating with mitotic chromatin. In this complex, L2 plays a crucial role in the interactions with cellular factors that enable delivery and ultimately tethering of the viral genome to mitotic chromatin. To date, the cellular proteins facilitating these steps remain unknown. Here, we addressed which cellular proteins may be required for this process. Using label-free mass spectrometry, biochemical assays, microscopy, and functional virological assays, we discovered that L2 engages a hitherto unknown protein complex of Ran-binding protein 10 (RanBP10), karyopherin alpha2 (KPNA2), and dynein light chain DYNLT3 to facilitate transport towards mitotic chromatin. Thus, our study not only identifies novel cellular interactors and mechanism that facilitate a poorly understood step in HPV entry, but also a novel cellular transport complex.
Identifiants
pubmed: 33974675
doi: 10.1371/journal.ppat.1009580
pii: PPATHOGENS-D-21-00411
pmc: PMC8139508
doi:
Substances chimiques
Capsid Proteins
0
Chromatin
0
DNA, Viral
0
Guanine Nucleotide Exchange Factors
0
KPNA2 protein, human
0
Microtubule-Associated Proteins
0
RANBP10 protein, human
0
alpha Karyopherins
0
DYNLT3 protein, human
EC 3.6.1.-
Dyneins
EC 3.6.4.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009580Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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